Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
mSphere. 2024 Oct 29;9(10):e0047124. doi: 10.1128/msphere.00471-24. Epub 2024 Oct 8.
is a phagocytic amoeba continuously eating, killing, and digesting bacteria. Previous studies have detected in cell extracts a bacteriolytic activity effective against bacteria. In this study, we characterized bacteriolytic activities found in cell extracts against five different bacteria (, , , , and ). We first analyzed the bacteriolytic activity against these five bacteria in parallel over a range of pH values. We then measured the remaining bacteriolytic activity in and knockout mutants. We also performed partial fractionation of extracts and assessed activity against different bacteria. Together our results indicate that optimal bacteriolytic activity against different bacteria results from the action of different effectors. Proteomic analysis allowed us to propose a list of potential bacteriolytic effectors.IMPORTANCEMany antibacterial effectors have been characterized over the past decades, and their biological importance, mode of action, and specificity are often still under study. Here we characterized bacteriolytic activity in extracts against five species of Gram-negative and Gram-positive bacteria. Our results reveal that optimal lysis of different bacteria mobilizes different effectors. Proteomic analysis generated a list of potential bacteriolytic effectors. This work opens the way for future analysis of the role of individual effectors in living cells.
是一种吞噬性变形虫，不断吞噬、杀死并消化细菌。先前的研究在 细胞提取物中检测到一种对 细菌有效的溶菌活性。在这项研究中，我们对 细胞提取物中针对五种不同细菌（ 、 、 、 和 ）的溶菌活性进行了表征。我们首先在一系列 pH 值下平行分析了这些五种细菌的溶菌活性。然后，我们在 和 敲除突变体中测量了剩余的溶菌活性。我们还对 提取物进行了部分分级，并评估了对不同细菌的活性。我们的研究结果表明，针对不同细菌的最佳溶菌活性来自不同效应物的作用。蛋白质组学分析使我们能够提出潜在的溶菌效应物列表。
在过去的几十年中，已经对许多抗菌效应物进行了表征，它们的生物学重要性、作用方式和特异性仍在研究中。在这里，我们对 提取物中针对五种革兰氏阴性和革兰氏阳性细菌的溶菌活性进行了表征。我们的结果表明，不同细菌的最佳裂解需要不同的效应物。蛋白质组学分析生成了潜在溶菌效应物的列表。这项工作为未来分析单个效应物在活 细胞中的作用开辟了道路。
