Effects of pasteurization temperature and amino acids on the gelation behavior of liquid egg yolk: Emphasizing rheology, gel properties, intermolecular forces and microstructure.

Pipeline blockage caused by liquid egg yolk (LEY) in the pasteurization process has become an urgent problem for egg industry. This study investigated the effects of amino acids (betaine/proline) on rheology of LEY and gel property of egg yolk gel (EYG) at various pasteurization temperatures (68, 72, and 76 °C). Rheological results revealed that 72 °C was the key transition point for increase in LEY thermal aggregation rate. Average particle size of EYG, BEYG and PEYG increased by 63.9 %, 27.3 % and 17.3 % with increasing pasteurization temperature. Amino acids promoted increase in disulfide bonding content and facilitated retention of free and bound water within gels. Moreover, amino acids enhanced crystallinity and order of gel structures. Amino acids can effectively mitigate thermal aggregation of LEY at mild temperatures and promote cross-linking of gel network at high temperatures. This study provides a theoretical foundation for heat resistance of LEY and application of EYG.