Benzhen Li, Shucheng Shao, Chenchang Bao, Zhaoxia Cui, Yanan Yang
School of Marine Science, Ningbo University, Ningbo, China.
School of Marine Science, Ningbo University, Ningbo, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2024 Dec;52:101334. doi: 10.1016/j.cbd.2024.101334. Epub 2024 Sep 28.
Prior to the pubertal molt and mating, the ovarian development of the mud crab Scylla paramamosain was primarily at stage II. However, immediately after mating, female crabs initiate vitellogenesis, and their ovaries quickly develop. The aim of this study was to identify differentially expressed genes associated with ovarian development in the mud crab before and after mating, in order to elucidate the influence of mating on ovarian development using comparative transcriptomics. The KEGG pathway analysis results indicated that ribosome and ribosome-related pathways were highly associated with ovarian development at stage II across both transcriptomes, likely to support the subsequent vitellogenesis by providing the necessary materials. Additionally, the neurodegeneration, MAPK, cAMP and PLD pathways were active in regulating oogonia differentiation, oocyte proliferation and vitellogenesis after mating. Meanwhile, certain intra-ovarian factors, such as the cell cycle-related genes cyclin B and APC, the forkhead box family genes Foxl2 and slp1, the SOX family gene SOX5-like, the hormone-related genes SULT1E1 and Eip74EF-like, the growth factor-related genes VEGFD-like and CUBE1-like, as well as HPS10 and tra1-like, have essential functions in regulating ovarian development after mating. Furthermore, the receptors of extra-ovarian hormones, such as RPCHR, HR4, and ILR1, as well as the neurotransmitter receptor 5-HTR4, were involved in ovarian development. It is believed that ovarian development is controlled by the coordinated action of both intrinsic and extrinsic endocrine factors, and these factors are influenced by mating. Finally, the analysis of epigenic modification-related genes, transcription factors, and target genes revealed the regulation of gene expression. Our study indicated that, those genes work in a coordinated manner to regulate the complex processes of follicle cell development, oogonia differentiation, oocyte proliferation, and vitellogenesis during ovarian development.
在青春期蜕皮和交配之前，拟穴青蟹的卵巢发育主要处于II期。然而，交配后雌蟹立即开始卵黄发生，其卵巢迅速发育。本研究的目的是鉴定拟穴青蟹交配前后与卵巢发育相关的差异表达基因，以便利用比较转录组学阐明交配对卵巢发育的影响。KEGG通路分析结果表明，核糖体和核糖体相关通路在两个转录组中均与II期卵巢发育高度相关，可能通过提供必要物质来支持随后的卵黄发生。此外，神经退行性变、MAPK、cAMP和PLD通路在交配后调节卵原细胞分化、卵母细胞增殖和卵黄发生中发挥作用。同时，某些卵巢内因子，如细胞周期相关基因细胞周期蛋白B和后期促进复合体，叉头框家族基因Foxl2和slp1，SOX家族基因SOX5-like，激素相关基因SULT1E1和Eip74EF-like，生长因子相关基因VEGFD-like和CUBE1-like，以及HPS10和tra1-like，在交配后调节卵巢发育中具有重要功能。此外，卵巢外激素的受体，如RPCHR、HR4和ILR1，以及神经递质受体5-HTR4，也参与了卵巢发育。据信，卵巢发育受内在和外在内分泌因子的协同作用控制，而这些因子受交配影响。最后，对表观遗传修饰相关基因、转录因子和靶基因的分析揭示了基因表达的调控。我们的研究表明，这些基因协同作用以调节卵巢发育过程中卵泡细胞发育、卵原细胞分化、卵母细胞增殖和卵黄发生的复杂过程。
