Devi Laxmi, Kushwaha Poonam, Ansari Tarique Mahmood, Rao Amit, Kumar Ashish
Faculty of Pharmacy, Integral University, Lucknow, India.
Smt.Vidyawati College of Pharmacy Jhansi, Uttar Pradesh, India.
Drug Res (Stuttg). 2025 Jan;75(1):34-44. doi: 10.1055/a-2418-2200. Epub 2024 Oct 8.
The targeted delivery of drugs is vital in breast cancer treatment due to its ability to produce long-lasting therapeutic effects with minimal side effects. This study reports the successful development of doxorubicin hydrochloride (DOX)-loaded colloidal gold nanoparticles stabilized with acacia gum (AG). Optimization studies varied AG concentrations (0.25% to 3% w/v) to determine optimal conditions for nanoparticle synthesis. The resulting acacia stabilized gold nanoparticles (AGNPs) were characterized using various techniques including high-resolution transmission electron microscopy (HR-TEM), powder X-ray diffraction (PXRD), differential scanning calorimetry (DSC), ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), and selected area electron diffraction (SAED). In vitro drug release studies demonstrated a higher release rate of DOX in sodium acetate buffer (pH 5.0) compared to phosphate buffer saline (pH 7.4), suggesting an enhanced therapeutic efficacy in acidic tumor environments. Cytotoxicity of DOX-AGNPs and free DOX was assessed in human breast cancer cells (MDA-MB-231). The DOX-AGNPs exhibited significantly greater cytotoxicity, indicating enhanced efficacy in targeting cancer cells. This enhancement suggests that adsorbing DOX on the surface of gold nanoparticles can improve drug delivery and effectiveness, potentially reducing side effects compared to pure DOX and traditional delivery methods. Stability tests conducted over six months at 25±1°C showed significant changes in particle size and PDI, suggesting limited stability under these conditions. Overall, the acacia-stabilized gold nanoparticles synthesized in this study exhibit promising characteristics for drug delivery applications, particularly in cancer therapy, with effective drug loading, controlled release, and favorable physicochemical properties.
由于药物靶向递送能够以最小的副作用产生持久的治疗效果，因此在乳腺癌治疗中至关重要。本研究报告了用阿拉伯胶（AG）稳定的载有盐酸多柔比星（DOX）的胶体金纳米颗粒的成功研发。优化研究改变了AG的浓度（0.25%至3% w/v），以确定纳米颗粒合成的最佳条件。使用包括高分辨率透射电子显微镜（HR-TEM）、粉末X射线衍射（PXRD）、差示扫描量热法（DSC）、紫外可见光谱、傅里叶变换红外光谱（FT-IR）、场发射扫描电子显微镜（FE-SEM）和选区电子衍射（SAED）等各种技术对所得的阿拉伯胶稳定的金纳米颗粒（AGNPs）进行了表征。体外药物释放研究表明，与磷酸盐缓冲盐水（pH 7.4）相比，DOX在醋酸钠缓冲液（pH 5.0）中的释放速率更高，这表明在酸性肿瘤环境中治疗效果增强。在人乳腺癌细胞（MDA-MB-231）中评估了DOX-AGNPs和游离DOX的细胞毒性。DOX-AGNPs表现出明显更大的细胞毒性，表明在靶向癌细胞方面疗效增强。这种增强表明，将DOX吸附在金纳米颗粒表面可以改善药物递送和有效性，与纯DOX和传统递送方法相比，可能减少副作用。在25±1°C下进行的为期六个月的稳定性测试显示，粒径和PDI有显著变化，表明在这些条件下稳定性有限。总体而言，本研究中合成的阿拉伯胶稳定的金纳米颗粒在药物递送应用中表现出有前景的特性，特别是在癌症治疗中，具有有效的药物负载、控释和良好的物理化学性质。
