Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province, 030801, People's Republic of China.
Infect Dis Poverty. 2024 Oct 9;13(1):73. doi: 10.1186/s40249-024-01246-9.
Toxocara canis, the most prevalent helminth in dogs and other canines, is one of the socioeconomically important zoonotic parasites, particularly affecting pediatric and adolescent populations in impoverished communities. However, limited information is available regarding the proteomes of female and male adult T. canis. To address this knowledge gap, we performed a comprehensive proteomic analysis to identify the proteins with differential abundance (PDAs) and gender-specifically expressed proteins between the two sexes adult T. canis.
The comparative proteomic analysis was carried out by the Orbitrap mass spectrometry (MS) with asymmetric track lossless (Astral) analyzer. The difference analysis was conducted using t-test and the proteins verification was achieved through parallel reaction monitoring (PRM). The potential biological functions of identified adult T. canis proteins and PDAs were predicted by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The domain, transcription factor and subcellular localization of the identified proteins and PDAs were analyzed by InterPro, AnimalTFDB 4.0 and Cell-mPLOC 2.0 databases, respectively.
A total of 8565 somatic proteins of adult T. canis were identified. Compared to male adult, 682 up-regulated PDAs and 844 down-regulated PDAs were identified in female adult with P-values < 0.05 and |log| > 1, including 139 proteins exclusively expressed in female and 272 proteins exclusively expressed in male. The GO annotation analysis using all PDAs revealed that the main biological processes, cellular components and molecular functions corresponded to aminoglycan metabolic process, extracellular region and protein tyrosine phosphatase activity, respectively. The KEGG analysis using all PDAs showed that the pathways were mainly associated with adipocytokine signaling pathway, proximal tubule bicarbonate reclamation and PPAR signaling pathway.
This study reveals the differential protein expression between female and male adult T. canis, providing valuable resource for developing the novel intervention strategies against T. canis infection in humans and animals, especially from the perspective of sexual development and reproduction.
犬弓首蛔虫是犬和其他犬科动物中最常见的寄生虫，是一种具有重要社会经济意义的人畜共患寄生虫，尤其影响贫困社区的儿科和青少年人群。然而，关于雌性和雄性成虫犬弓首蛔虫的蛋白质组信息有限。为了解决这一知识空白，我们进行了全面的蛋白质组学分析，以鉴定雌雄成虫犬弓首蛔虫之间具有差异丰度（PDAs）和性别特异性表达的蛋白质。
采用 Orbitrap 质谱（MS）和不对称轨道损耗（Astral）分析器进行比较蛋白质组学分析。采用 t 检验进行差异分析，通过平行反应监测（PRM）进行蛋白质验证。通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）数据库预测鉴定的成虫犬弓首蛔虫蛋白和 PDAs 的潜在生物学功能。通过 InterPro、AnimalTFDB 4.0 和 Cell-mPLOC 2.0 数据库分别分析鉴定蛋白和 PDAs 的结构域、转录因子和亚细胞定位。
鉴定出成虫犬弓首蛔虫的 8565 个体蛋白。与雄性成虫相比，雌性成虫中鉴定出 682 个上调 PDAs 和 844 个下调 PDAs，P 值<0.05，|log|>1，其中 139 个蛋白仅在雌性中表达，272 个蛋白仅在雄性中表达。使用所有 PDAs 进行的 GO 注释分析表明，主要的生物学过程、细胞成分和分子功能分别对应于氨基糖代谢过程、细胞外区和蛋白酪氨酸磷酸酶活性。使用所有 PDAs 进行的 KEGG 分析表明，途径主要与脂肪细胞因子信号通路、近端肾小管碳酸氢盐回收和 PPAR 信号通路相关。
本研究揭示了雌性和雄性成虫犬弓首蛔虫之间的差异蛋白表达，为开发针对人和动物犬弓首蛔虫感染的新型干预策略提供了有价值的资源，特别是从性发育和生殖的角度。
Zotero 插件
