Xu Chengxuan, Qian Lijuan, Meng Qi, Meng Xiangru, Sun Yujun
College of Agriculture, Anhui Science and Technology University, Fengyang, 233100, China.
College of Biomedicine and Health, Anhui Science and Technology University, Fengyang, 233100, China.
Sci Rep. 2025 Jul 26;15(1):27285. doi: 10.1038/s41598-025-13343-2.
This study investigates the effects of sodium selenite on the yield, selenium content, and proteomic profile of Pleurotus pulmonarius (P. pulmonarius). Treatment with 2 µg/mL sodium selenite (PP-Se2) resulted in a 17.87% increase in the yield of P. pulmonarius. In contrast, treatment with 10 µg/mL sodium selenate (PP-Se10) caused a 23.58% reduction in yield. The selenium content exhibited a positive correlation with the sodium selenite concentration in the cultivation substrate. Based on data-independent acquisition (DIA) proteomics analysis, it was revealed that PP-Se2 and PP-Se10 contained 141 differential expressed proteins (DEPs) (64 up-regulated and 77 down-regulated) and 129 DEPs (73 up-regulated and 56 down-regulated), respectively. DEPs in PP-Se2 were predominantly involved in carbon metabolism, ribosomes, peroxisomes, and ABC transporters. In contrast, DEPs in PP-Se10 were primarily enriched in the biosynthesis of secondary metabolites and cysteine and methionine metabolism. These results suggest that P. pulmonarius exhibits distinct metabolic response mechanisms to varying levels of selenium treatment, with these pathways potentially being influenced by selenium concentration. These findings help to fill the gap in selenium proteomics research in edible fungi and provide new theoretical insights for the development of selenium-enriched functional foods.
本研究调查了亚硒酸钠对肺形侧耳产量、硒含量和蛋白质组图谱的影响。用2μg/mL亚硒酸钠处理（PP-Se2）使肺形侧耳产量提高了17.87%。相比之下，用10μg/mL硒酸钠处理（PP-Se10）导致产量降低了23.58%。硒含量与栽培基质中亚硒酸钠浓度呈正相关。基于数据非依赖采集（DIA）蛋白质组学分析发现，PP-Se2和PP-Se10分别包含141个差异表达蛋白（DEP）（64个上调和77个下调）和129个DEP（73个上调和56个下调）。PP-Se2中的DEP主要参与碳代谢、核糖体、过氧化物酶体和ABC转运蛋白。相比之下，PP-Se10中的DEP主要富集在次生代谢物生物合成以及半胱氨酸和蛋氨酸代谢中。这些结果表明，肺形侧耳对不同水平的硒处理表现出不同的代谢反应机制，这些途径可能受硒浓度影响。这些发现有助于填补食用菌硒蛋白质组学研究的空白，并为富硒功能食品的开发提供新的理论见解。
