Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 01797, Korea.
Arch Microbiol. 2024 Oct 9;206(11):428. doi: 10.1007/s00203-024-04150-w.
Strains M29 and ASUV-10-1, which are aerobic, non-flagellated, and Gram-stain-negative, were isolated from soil samples collected in Inje (37°57'49.1"N 128°19'53.7"E) and Cheonan City (36°48'47.1"N 127°05'22.4"E), South Korea. Phylogenetic analyses based on rRNA gene sequences revealed that strains M29 and ASUV-10-1 form a distinct branch within the family Hymenobacter (order Cytophagales, class Cytophagia). Strain M29 is most closely related to Hymenobacter rubidus DG7B with a 16 S rRNA gene sequence similarity of 97.05%. Strain ASUV-10-1 shows closest genetic similarity to Hymenobacter frigidus B1789 (96.42%), Hymenobacter jeongseonensis BT683 (95.97%), and Hymenobacter terricola 3F2T (95.65%). The optimal growth conditions for these strains are pH 7.0, no NaCl, and a temperature of 25 °C. The dominant cellular fatty acids identified in these strains are iso-C, anteiso-C, and Summed Feature 3 (Cω 7c / Cω 6c). Both strains predominantly contain MK-7 as the respiratory quinone. The major polar lipids in strains M29 and ASUV-10-1 are phosphatidylethanolamine, aminophospholipid, and aminolipid. Based on biochemical, chemotaxonomic, and phylogenetic data, it is evident that M29 and ASUV-10-1 represent new species within the genus Hymenobacter. The new species were classified based on biochemical and chemotaxonomic characteristics. The taxonomic classification of these species was conducted following the guidelines and protocols outlined in Bergey's Manual of Systematic Bacteriology. We followed the methods for determining physiological and biochemical characteristics, as well as chemotaxonomic markers such as fatty acid profiles, quinone types, and polar lipid compositions. We also compared with the results of carbohydrate utilization and enzyme activities results [Bergey 1994]. Therefore, we propose the names Hymenobacter mellowenesis for strain M29 (= KCTC 102056 = NBRC 116578) and Hymenobacter aranciens for strain ASUV-10-1 (= KCTC 92969 = NBRC 116575).
菌株 M29 和 ASUV-10-1 是从韩国晋州（37°57'49.1"N 128°19'53.7"E）和天安市（36°48'47.1"N 127°05'22.4"E）的土壤样本中分离得到的，它们是需氧、无鞭毛和革兰氏阴性菌。基于 rRNA 基因序列的系统发育分析表明，菌株 M29 和 ASUV-10-1 在海杆菌科（Cytophagales 目，Cytophagia 纲）内形成一个独特的分支。菌株 M29 与 Hymenobacter rubidus DG7B 的 16S rRNA 基因序列相似度最高，为 97.05%。菌株 ASUV-10-1 与 Hymenobacter frigidus B1789（96.42%）、Hymenobacter jeongseonensis BT683（95.97%）和 Hymenobacter terricola 3F2T（95.65%）的遗传相似度最近。这些菌株的最佳生长条件为 pH 值 7.0、无 NaCl 和温度 25°C。在这些菌株中鉴定到的主要细胞脂肪酸是 iso-C、anteiso-C 和总和特征 3（Cω 7c / Cω 6c）。两个菌株都主要含有 MK-7 作为呼吸醌。菌株 M29 和 ASUV-10-1 中的主要极性脂是磷脂酰乙醇胺、氨基磷脂和氨基脂。基于生化、化学分类和系统发育数据，M29 和 ASUV-10-1 显然代表了海杆菌属中的新种。新种是根据生化和化学分类特征分类的。这些种的分类是根据 Bergey 的《系统细菌学手册》中概述的指南和协议进行的。我们遵循了确定生理生化特征以及化学分类标记（如脂肪酸图谱、醌型和极性脂组成）的方法。我们还将结果与碳水化合物利用和酶活性结果进行了比较 [Bergey 1994]。因此，我们提议将菌株 M29（=KCTC 102056=NRBC 116578）命名为 Hymenobacter mellowenesis，将菌株 ASUV-10-1（=KCTC 92969=NRBC 116575）命名为 Hymenobacter aranciens。
