Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon, 22012, Republic of Korea.
Research Group of Healthcare, Korea Food Research Institute, Wanju, 55365, Republic of Korea.
Antonie Van Leeuwenhoek. 2020 Jul;113(7):947-957. doi: 10.1007/s10482-020-01402-9. Epub 2020 Mar 28.
A red-pigmented bacterial strain, designated KIGAM108, within the family Hymenobacteraceae was isolated from zeolite in the Gampo-41 mine of the Gyeongju, Republic of Korea. This strain was a Gram-negative, strictly aerobic, non-spore forming, rod-shaped bacterium. Phylogenetic analysis of the 16S rRNA gene sequence of strain KIGAM108 found that it was related to the genus Hymenobacter, with similarities of 96.6, 96.4, 95.5, and 95.0% to H. fastidiosus VUG-A124, H. algoricola VUG-A23a, H. crusticola MIMBbqt21, and H. daecheongensis DSM 21074, respectively. Strain KIGAM108 grew in the presence of 0-0.5% (w/v) NaCl at 4-37 °C and pH 6.0-10.0. This isolate contained MK-7 as a respiratory quinone. The polar lipids of strain KIGAM108 were identified as phosphatidylethanolamine, two unidentified aminophospholipids, one unidentified phospholipid and five unidentified lipids. The major fatty acids profile showed summed feature 3 (Cω6c and/or Cω7c) (22.3%), anteiso-C (17.1%), Cω5c (13.3%), and iso-C (11.0%). The genomic DNA G + C content was 60.0 mol%. Based on the polyphasic taxonomic data, strain KIGAM108 is considered to represent a novel species of the genus Hymenobacter, for which the name Hymenobacter lutimineralis sp. nov. is proposed. The type strain is KIGAM108 (=KCTC 72263 =JCM 33444).
一株红色着色的细菌菌株，被命名为 KIGAM108，属于 Hymenobacteraceae 科，是从韩国庆州 Gampo-41 矿沸石中分离出来的。该菌株是革兰氏阴性、严格需氧、非孢子形成、杆状细菌。通过对 KIGAM108 菌株的 16S rRNA 基因序列进行系统发育分析，发现它与 Hymenobacter 属有关，与 Hymenobacter fastidiosus VUG-A124、Hymenobacter algoricola VUG-A23a、Hymenobacter crusticola MIMBbqt21 和 Hymenobacter daecheongensis DSM 21074 的相似度分别为 96.6%、96.4%、95.5%和 95.0%。KIGAM108 菌株在 0-0.5%（w/v）NaCl 浓度、4-37°C 和 pH 值 6.0-10.0 的条件下生长。该分离株含有 MK-7 作为呼吸醌。KIGAM108 菌株的极性脂类鉴定为磷脂酰乙醇胺、两种未鉴定的氨基磷脂、一种未鉴定的磷脂和五种未鉴定的脂类。主要脂肪酸图谱显示，特征脂肪酸 3（Cω6c 和/或 Cω7c）（22.3%）、anteiso-C（17.1%）、Cω5c（13.3%）和 iso-C（11.0%）。基因组 DNA G+C 含量为 60.0 mol%。基于多相分类学数据，KIGAM108 菌株被认为代表了 Hymenobacter 属的一个新种，现提议将其命名为 Lutimineralis lutimineralis sp. nov.。模式菌株为 KIGAM108（=KCTC 72263=JCM 33444）。
