Molecular Biogeochemistry Laboratory, Biological & Genetic Resources Institute (BGRI), Sejong, Republic of Korea.
Department of Bioenvironmental Chemistry, Jeonbuk National University, Jeonju, Republic of Korea.
Environ Geochem Health. 2024 Oct 9;46(11):470. doi: 10.1007/s10653-024-02251-5.
The detoxification process of transforming arsenite (As(III)) to arsenate (As(V)) through bacterial oxidation presents a potent approach for bioremediation of arsenic-polluted soils in abandoned mines. In this study, twelve indigenous arsenic-oxidizing bacteria (AOB) were isolated from arsenic-contaminated soils. Among these, Paenibacillus xylanexedens EBC-SK As2 (MF928871) and Ochrobactrum anthropi EBC-SK As11 (MF928880) were identified as the most effective arsenic-oxidizing isolates. Evaluations for bacterial arsenic resistance demonstrated that P. xylanexedens EBC-SK As2 (MF928871) could resist As(III) up to 40 mM, while O. anthropi EBC-SK As11 (MF928880) could resist As(III) up to 25 mM. From these bacterial strains, genotypes of arsenic resistance system (ars) were detected, encompassing ars leader genes (arsR and arsD), membrane genes (arsB and arsJ), and aox genes known to be crucial for arsenic detoxification. These ars genotypes in the isolated AOBs might play an instrumental role in arsenic-contaminated soils with potential to reduce arsenic contamination.
通过细菌氧化将亚砷酸盐（As(III)）转化为砷酸盐（As(V)）的解毒过程为受污染矿山废弃地的砷污染土壤的生物修复提供了一种有效方法。在这项研究中，从砷污染土壤中分离出了十二株本土砷氧化细菌（AOB）。其中，解木聚糖芽孢杆菌 EBC-SK As2（MF928871）和人苍白杆菌 EBC-SK As11（MF928880）被鉴定为最有效的砷氧化分离株。细菌砷抗性评估表明，解木聚糖芽孢杆菌 EBC-SK As2（MF928871）可耐受高达 40mM 的 As(III)，而人苍白杆菌 EBC-SK As11（MF928880）可耐受高达 25mM 的 As(III)。从这些细菌菌株中，检测到了砷抗性系统（ars）的基因型，包括 ars 启动基因（arsR 和 arsD）、膜基因（arsB 和 arsJ）以及已知对砷解毒至关重要的 aox 基因。这些分离的 AOB 中的 ars 基因型可能在潜在降低砷污染的砷污染土壤中发挥重要作用。
