Molecular Biogeochemistry Laboratory, Biological & Genetic Resources Institute (BGRI), 505 Inno-Biz Park, 1646 Yuseong-daero, Yeseong-gu, Daejeon 305-811, Republic of Korea.
Environ Pollut. 2015 Nov;206:315-23. doi: 10.1016/j.envpol.2015.07.027. Epub 2015 Jul 25.
The potential arsenite bioteansformation activity of arsenic was investigated by examining bacterial arsenic arsenite-oxidizing gene such as aoxS, aoxR, aoxA, aoxB, aoxC, and aoxD in high arsenic-contaminated drinking water produced from the surface water of floating houses. There is a biogeochemical cycle of activity involving arsenite oxidase aox system and the ars (arsenic resistance system) gene operon and aoxR leader gene activity in Alcaligenes faecalis SRR-11 and aoxS leader gene activity in Achromobacter xylosoxidans TSL-66. Batch experiments showed that SRR-11 and TSL-66 completely oxidized 1 mM of As (III) to As (V) within 35-40 h. The leaders of aoxS and aoxR are important for gene activity, and their effects in arsenic bioremediation and mobility in natural water has a significant ecological role because it allows arsenite oxidase in bacteria to control the biogeochemical cycle of arsenic-contaminated drinking water produced from surface water of floating houses.
研究了砷的亚砷酸盐生物转化活性，方法是检测来自浮屋地表水的高砷污染饮用水中的细菌砷亚砷酸盐氧化基因，如 aoxS、aoxR、aoxA、aoxB、aoxC 和 aoxD。在活性的生物地球化学循环中，涉及亚砷酸盐氧化酶 aox 系统和 ars（砷抗性系统）基因操纵子以及 Alcaligenes faecalis SRR-11 中的 aoxR 启动子基因活性和 Achromobacter xylosoxidans TSL-66 中的 aoxS 启动子基因活性。批量实验表明，SRR-11 和 TSL-66 在 35-40 小时内将 1mM 的 As(III)完全氧化为 As(V)。aoxS 和 aoxR 的启动子对基因活性很重要，它们在砷的生物修复和自然水中的迁移中的作用具有重要的生态作用，因为它允许细菌中的亚砷酸盐氧化酶控制来自浮屋地表水的砷污染饮用水的生物地球化学循环。
