Guérin M, Lacombe M J, Riou G
C R Acad Sci III. 1985;301(20):833-8.
The c-myc oncogene was characterized and its expression analyzed in 32 mammary adenocarcinomas and in 2 benign breast tumors from 34 untreated patients. Southern blot hybridization experiments have demonstrated the amplification of the oncogene (3 to 30 fold) in 3 carcinomas. The analysis of total RNA by Northern blot revealed the presence of a 2.4 kb c-myc RNA band. In 7 out of 10 carcinomas from patients with 3 or more than 3 lymph node metastases the level of c-myc expression evaluated by dot blot analysis was 4 to 14 fold greater than that of normal human tissues. In only 5 out of 22 carcinomas from patients without lymph node metastases or less than 3 invaded nodes the level of c-myc expression was also higher (4 to 10 fold). The level of c-myc expression was not significantly enhanced in the 2 benign tumors. It is suggested that the c-myc gene activation could be associated to a higher degree of malignancy of mammary carcinomas.
对34例未经治疗患者的32例乳腺腺癌和2例乳腺良性肿瘤进行了c-myc癌基因特征分析及其表达分析。Southern印迹杂交实验已证实3例癌中该癌基因发生扩增（3至30倍）。Northern印迹法对总RNA的分析显示存在一条2.4 kb的c-myc RNA条带。在有3个或3个以上淋巴结转移患者的10例癌中，有7例通过斑点印迹分析评估的c-myc表达水平比正常人体组织高4至14倍。在无淋巴结转移或侵袭淋巴结少于3个患者的22例癌中，只有5例c-myc表达水平也较高（4至10倍）。2例良性肿瘤中c-myc表达水平未显著增强。提示c-myc基因激活可能与乳腺癌更高的恶性程度相关。
