Programa de Pós-graduação em Ciências Ambientais, Universidade Federal do Amapá - UFAP, Macapá, AP, Brasil.
Programa de Pós-graduação em Saúde e Produção Animal na Amazônia, Universidade Federal Rural da Amazônia - UFRA, Belém, PA, Brasil.
Rev Bras Parasitol Vet. 2024 Oct 7;33(3):e011024. doi: 10.1590/S1984-29612024058. eCollection 2024.
Myxozoa is a class of the Phylum Cnidaria made up of endoparasites from aquatic habitats. The genus Ceratomyxa preferentially infects marine fish, with the gallbladder being the main site parasitized. This study aimed to describe a new species of Ceratomyxa found in this organ in Boulengerella cuvieri using morphological, morphometric characterization and phylogenetic analysis of 18S rDNA gene sequences. Specimens of B. cuvieri were collected, anesthetized, desensitized and biometric measurements were performed. The organs were analyzed under a stereomicroscope and fragments of internal organs were extracted for light microscopy analysis, preserved in 80% ethanol for 18S rDNA gene analysis and fixed in Davidson solution for histological processing. Free spores of Ceratomyxa were observed in the gallbladder, in plasmodia with wave-like movements, with the following dimensions: spore width (24.5 ± 0.4) µm, spore length (5.2 ± 0.3) µm, polar capsule width (1.8 ± 0.2) µm, polar capsule length (2.1 ± 0.3) µm, number of polar tubule turns (4-5) and 100% prevalence. Phylogenetic analysis confirmed that Ceratomyxa matosi n. sp. is a new species, grouped with other freshwater Ceratomyxa species from the Amazon, representing the second description of species of this genus in the state of Amapá.
Myxozoa 是刺胞动物门的一个类群，由水生栖息地的内寄生动物组成。 Ceratomyxa 属优先感染海洋鱼类，胆囊是主要寄生部位。本研究旨在描述在 Boulengerella cuvieri 这种器官中发现的一种新的 Ceratomyxa 物种，使用形态学、形态计量学特征和 18S rDNA 基因序列的系统发育分析。收集了 B. cuvieri 的标本，进行麻醉、脱敏和生物计量测量。在立体显微镜下分析器官，提取内脏片段进行光镜分析，保存在 80%乙醇中用于 18S rDNA 基因分析，并固定在 Davidson 溶液中进行组织学处理。在胆囊中观察到 Ceratomyxa 的自由孢子，在具有波浪状运动的原生动物中，具有以下尺寸：孢子宽度（24.5 ± 0.4）µm，孢子长度（5.2 ± 0.3）µm，极囊宽度（1.8 ± 0.2）µm，极囊长度（2.1 ± 0.3）µm，极囊管匝数（4-5）和 100%的流行率。系统发育分析证实，Ceratomyxa matosi n. sp. 是一个新物种，与亚马逊地区的其他淡水 Ceratomyxa 物种聚在一起，这是该属在阿马帕州的第二个物种描述。
