Liu Qing, Zhang Ye, Yu Shuxian, Zhao Chuanze, Yang Yuqing, Yan Jianyu, Wang Yuge, Liu Dayong, Liu Ying, Zhang Xu
Department of Endodontics, Tianjin Medical University School and Hospital of Stomatology & Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, No. 12 Qixiangtai Road, Heping District, Tianjin 300070, China.
Department of Endodontics, Tianjin Medical University School and Hospital of Stomatology & Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, No. 12 Qixiangtai Road, Heping District, Tianjin 300070, China; Department of Biomaterials, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan 250012, China.
Colloids Surf B Biointerfaces. 2025 Jan;245:114290. doi: 10.1016/j.colsurfb.2024.114290. Epub 2024 Oct 2.
Proteoglycans (PG) is crucial for regulating collagen formation and mineralization during bone tissue development. A wide variety of PG-modified collagen scaffolds have been proposed for bone engineering application to promote biological responses and work as artificial matrices that guide tissue regeneration. However, poor performance of theses biomaterials against infections has led to an unmet need for clinical prevention. Therefore, we utilized proanthocyanidins (PA) to simulate the functions of PG, including mediating the collagen assembly and intrafibrillar mineralization, to optimize scaffolds performance. The excellent antibacterial properties of PA can endow the scaffolds with anti-infection effects in the process of tissue regeneration. When PA was added during fibrillogenesis, the collagen fibrils appeared irregular aggregation and the mineralization degree was reduced. In contrast, the addition of PA after collagen self-assembly improved the latter's ability to act as a deposition template and remarkably promoted mineral ions infiltration, thus enhancing intrafibrillar mineralization. The PA-modified scaffold displayed a highly hydrophilicity behaviour and long-term resistance to degradation. The sustained release of PA effectively inhibited the activity of Staphylococcus aureus. The scaffold also showed excellent biocompatibility and improved bone regeneration in calvarial critical-size defect models. The application of PA enables a dual-function scaffold with favourable intrafibrillar mineralization and anti-bacterial properties for bone regeneration.
蛋白聚糖（PG）在骨组织发育过程中对调节胶原蛋白形成和矿化至关重要。多种PG修饰的胶原蛋白支架已被提出用于骨工程应用，以促进生物反应并作为引导组织再生的人工基质。然而，这些生物材料抗感染性能不佳导致临床预防需求未得到满足。因此，我们利用原花青素（PA）模拟PG的功能，包括介导胶原蛋白组装和纤维内矿化，以优化支架性能。PA的优异抗菌性能可使支架在组织再生过程中具有抗感染作用。在原纤维形成过程中添加PA时，胶原纤维出现不规则聚集且矿化程度降低。相反，在胶原蛋白自组装后添加PA提高了其作为沉积模板的能力，并显著促进了矿质离子渗透，从而增强了纤维内矿化。PA修饰的支架表现出高度亲水性行为和长期抗降解性。PA的持续释放有效抑制了金黄色葡萄球菌的活性。该支架在颅骨临界尺寸缺损模型中还表现出优异的生物相容性并促进了骨再生。PA的应用使一种具有良好纤维内矿化和抗菌性能的双功能支架用于骨再生成为可能。
