Oral Pathology Department, Faculty of Dentistry, Tanta University, El-Gesh Street, Tanta, 31527, Egypt; Department of Biomedical Dental Sciences, Faculty of Dentistry, Al-Baha University, Al-Baha, 65511, Saudi Arabia.
Department of Preventive Dental Sciences, Faculty of Dentistry, Al-Baha University, Al-Baha, Saudi Arabia.
Pathol Res Pract. 2024 Nov;263:155639. doi: 10.1016/j.prp.2024.155639. Epub 2024 Oct 6.
BACKGROUND / PURPOSE: The progression of epithelial to mesenchymal tissue (EMT) is a highly intricate process that facilitates the transformation of cancer cells, allowing them to changeover their characteristic epithelial properties to mesenchymal attributes. This notable change empowers the cells with enhanced mobility and the ability to migrate to distant locations. Furthermore, it is imperative to adopt the idea of macrophage tumor cell fusion to achieve comprehensive considerate of this phenomenon. Our primary objective was to conduct a thorough investigation of macrophage-restricted antigens expression, specifically CD68 and CD163, in malignant epithelial cells of oral cavity squamous cell carcinoma (OSCC) to elucidate aforementioned perceptions.
CD68 and CD163 immunohistochemical expression were assessed in oral squamous cell carcinoma (OSCC), encompassing both the neoplastic cells and the tumor-associated macrophages (TAMs).
Both CD68 and CD163 antigens were revealed in OSCC malignant epithelial cells in a granular cell pattern, localized in membrane and cytoplasm of tumor cells respectively as well as in the infiltrating TAMs.
The macrophage antigens were not limited to the infiltrating tumor-associated macrophages (TAMs), but were also observed in a substantial proportion of OSCC malignant epithelial cells within the tumor parenchyma. This particular expression pattern may represent a subset of tumor cells that have undergone an epithelial to a mesenchymal phenotypic transition. In addition, fusion of macrophages with tumor cells cannot be excluded; both might be associated with increased metastatic activity of OSCC.
背景/目的：上皮到间充质组织（EMT）的进展是一个高度复杂的过程，它促进了癌细胞的转化，使它们能够改变其特征性的上皮特性为间充质特性。这种显著的变化赋予了细胞更高的迁移能力和迁移到远处的能力。此外，必须采用巨噬细胞-肿瘤细胞融合的概念来全面考虑这一现象。我们的主要目的是深入研究口腔鳞状细胞癌（OSCC）恶性上皮细胞中巨噬细胞受限抗原的表达，特别是 CD68 和 CD163，以阐明上述观点。
对口腔鳞状细胞癌（OSCC）中的 CD68 和 CD163 免疫组织化学表达进行评估，包括肿瘤细胞和肿瘤相关巨噬细胞（TAMs）。
CD68 和 CD163 抗原均在 OSCC 恶性上皮细胞中呈颗粒状细胞模式表达，分别定位于肿瘤细胞的膜和细胞质以及浸润的 TAMs 中。
巨噬细胞抗原不仅局限于浸润的肿瘤相关巨噬细胞（TAMs），而且在肿瘤实质内的相当一部分 OSCC 恶性上皮细胞中也有表达。这种特殊的表达模式可能代表了经历上皮到间充质表型转化的肿瘤细胞亚群。此外，不能排除巨噬细胞与肿瘤细胞的融合；两者都可能与 OSCC 转移活性的增加有关。
