Fujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Fujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Virology. 2024 Dec;600:110252. doi: 10.1016/j.virol.2024.110252. Epub 2024 Sep 26.
The genome of a monopartite begomovirus, or the DNA-A component of a bipartite begomovirus, typically encodes six proteins: two on the viral strand (AV1/V1 and AV2/V2) and four on the complementary strand (AC1/C1, AC2/C2, AC3/C3, AC4/C4). Recent studies, however, have identified additional begomoviral proteins with various functions. This paper reports that euphorbia leaf curl virus (EuLCV), a monopartite begomovirus, encodes a seventh protein, C5. Promoter activity of the upstream fragment of the EuLCV C5 gene was shown using a GUS expression vector. EuLCV C5 also enhanced the pathogenicity and accumulation of potato virus X (PVX) in Nicotiana benthamiana. Localization studies revealed that EuLCV C5 localizes to the cytoplasm and nucleus, forming granular structures on the cell membrane. Additionally, C5 acts as a post-transcriptional gene silencing (PTGS) suppressor. A C5 deletion mutant of EuLCV (EuLCV-ΔC5) exhibited reduced pathogenicity and viral accumulation compared to wild-type EuLCV in N. benthamiana.
单分体番茄黄曲叶病毒或二分体番茄黄曲叶病毒的 DNA-A 组分通常编码六个蛋白：两个在病毒链上（AV1/V1 和 AV2/V2），四个在互补链上（AC1/C1、AC2/C2、AC3/C3、AC4/C4）。然而，最近的研究发现了具有各种功能的额外的黄曲叶病毒蛋白。本文报道了单分体番茄黄曲叶病毒（EuLCV）编码第七个蛋白 C5。使用 GUS 表达载体显示了 EuLCV C5 基因上游片段的启动子活性。EuLCV C5 还增强了 PVX 在 Nicotiana benthamiana 中的致病性和积累。定位研究表明，EuLCV C5 定位于细胞质和细胞核，在细胞膜上形成颗粒状结构。此外，C5 作为转录后基因沉默（PTGS）抑制剂发挥作用。与野生型 EuLCV 相比，EuLCV 的 C5 缺失突变体（EuLCV-ΔC5）在 N. benthamiana 中的致病性和病毒积累降低。
