Department of Blood Transfusion, The Affiliated Hospital of Guizhou Medical University, Guiyang 550001, China; Department of Clinical Hematology, School of Clinical Laboratory Science, Guizhou Medical University, Guiyang 550001, China.
The Collaborative Innovation Center for Eco-Friendly and Fire-Safety Polymeric Materials (MoE), National Engineering Laboratory of Eco-Friendly Polymeric Materials, (Sichuan), State Key Laboratory of Polymer Materials Engineering, College of Chemistry, Sichuan University, Chengdu 610064, China.
Int J Biol Macromol. 2024 Nov;281(Pt 2):136209. doi: 10.1016/j.ijbiomac.2024.136209. Epub 2024 Oct 8.
Platelet-rich plasma (PRP) has been recognized as an effective therapy in regenerative medicine and surgery, which can reduce the risk of antibiotic abuse and promote the healing of infected wounds. Recent advances in PRP-based treatments have focused on the controlled release of growth factors in PRP with biocompatible hydrogels and antimicrobial promotion by introducing hydrogel components or antibiotics, while the inherent antimicrobial activity of PRP is mostly neglected or sacrificed. Here, we demonstrate the combination of an antimicrobial polysaccharide, carboxymethyl chitosan, and PRP to construct an antimicrobial hydrogel via dynamic bonding with oxidized chondroitin sulfate. Significant inhibitory effects against Staphylococcus aureus and Escherichia coli (95 % of inhibition rate) are achieved through the synergistic contributions of the polysaccharide and PRP. Additionally, the resulting hydrogel promotes the migration of NIH-3T3 fibroblasts and collagen deposition by approximately 1.7 and 1.8 times, respectively, thereby accelerating the healing process of infected wounds. This work may bring new perspectives for potent applications of PRP-based hydrogel dressings for antibiotic-free management of infected wounds.
富含血小板的血浆 (PRP) 已被认为是再生医学和外科手术中的一种有效治疗方法，它可以降低抗生素滥用的风险，促进感染伤口的愈合。最近，基于 PRP 的治疗方法的进展集中在通过生物相容性水凝胶控制 PRP 中生长因子的释放，并通过引入水凝胶成分或抗生素来促进抗菌作用，而 PRP 的固有抗菌活性在很大程度上被忽视或牺牲。在这里，我们展示了一种抗菌多糖羧甲基壳聚糖与 PRP 的结合，通过与氧化硫酸软骨素的动态键合来构建一种抗菌水凝胶。多糖和 PRP 的协同作用实现了对金黄色葡萄球菌和大肠杆菌的显著抑制作用（抑制率达 95%）。此外，所得水凝胶分别将 NIH-3T3 成纤维细胞的迁移和胶原蛋白沉积促进约 1.7 倍和 1.8 倍，从而加速感染伤口的愈合过程。这项工作可能为基于 PRP 的水凝胶敷料在无抗生素管理感染伤口方面的有效应用带来新的视角。
