病毒 DNA 聚合酶 ORF30 中单个氨基酸突变（D752N）导致神经致病性马疱疹病毒 1 型 Ab4p 在仓鼠中的衰减。

Attenuation of the neuropathogenic equine herpesvirus type 1 strain Ab4p in hamsters by a single amino acid mutation (D752N) in viral DNA polymerase ORF30.

机构信息

Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Joint Department of Veterinary Medicine, Gifu University, Gifu, Japan.

The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.

出版信息

J Vet Med Sci. 2024 Dec 1;86(12):1273-1278. doi: 10.1292/jvms.24-0338. Epub 2024 Oct 9.

DOI:10.1292/jvms.24-0338

PMID:39384384

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11612248/

Abstract

Equine herpesvirus type 1 (EHV-1) causes abortion, respiratory infection, and neurological diseases (equine herpesvirus myeloencephalopathy) in horses. A single nucleotide polymorphism (SNP) associated with a single amino acid in the DNA polymerase gene (ORF30, in which D752 is neuropathogenic and N752 is non-neuropathogenic) of EHV-1 has been associated with neuropathogenicity in horses. We constructed an EHV-1 Ab4p ORF30 N752 mutant and a repair virus to examine the effect of a D752N mutation on the neuropathogenicity of the virus in Syrian hamsters. The N752 mutation did not affect viral growth in cultured cells but it did attenuate the neuropathogenicity of Ab4p in the hamsters. The results suggest that D752N is involved in neuropathogenicity not only in horses but also in hamsters.

摘要

马疱疹病毒 1 型（EHV-1）可引起马流产、呼吸道感染和神经系统疾病（马疱疹病毒脑脊髓炎）。EHV-1 的 DNA 聚合酶基因（ORF30）中与单个氨基酸相关的单核苷酸多态性（SNP）与病毒的神经致病性有关（其中 D752 是神经致病性的，N752 是非神经致病性的）。我们构建了 EHV-1 Ab4p ORF30 N752 突变体和修复病毒，以研究 D752N 突变对病毒在叙利亚仓鼠中的神经致病性的影响。N752 突变不影响病毒在培养细胞中的生长，但可削弱 Ab4p 在仓鼠中的神经致病性。结果表明，D752N 不仅参与了马的神经致病性，也参与了仓鼠的神经致病性。