Graf L, Koch N, Schirrmacher V
Mol Immunol. 1985 Dec;22(12):1371-7. doi: 10.1016/0161-5890(85)90059-8.
ESb, a cellular high metastatic variant derived from the murine T-cell lymphoma L5178Y (Eb), was found to synthesize Ia antigens. Ia-specific antibodies reacted with the ESb cells and precipitated Ia-like molecules from them. Two-dimensional gel electrophoretic analysis of immunoprecipitates of metabolically labeled ESb cells indicated that the Ia molecules on ESb were indistinguishable from those on murine B-cells. No Ia antigens were detectable on the parental tumor line Eb. Treatment with recombinant interferon-gamma (IFN-gamma) caused enhancement of class I histocompatibility antigen expression on Eb and ESb tumor lines. In ESb cells the expression of Ia and of Ia-associated invariant chain (Ii) was also increased upon IFN-gamma treatment. No induction of either Ia and Ii antigens was observed upon IFN-gamma treatment of the Eb line. These studies demonstrate a substantial difference between the Eb and ESb tumor lines with respect to: (i) constitutive expression of class II major histocompatibility antigens, and (ii) response to IFN-gamma treatment.
ESb是一种从鼠T细胞淋巴瘤L5178Y（Eb）衍生而来的细胞高转移变体，被发现能合成Ia抗原。Ia特异性抗体与ESb细胞发生反应，并从其中沉淀出Ia样分子。对代谢标记的ESb细胞免疫沉淀物进行的二维凝胶电泳分析表明，ESb上的Ia分子与鼠B细胞上的Ia分子无法区分。在亲代肿瘤细胞系Eb上未检测到Ia抗原。用重组干扰素-γ（IFN-γ）处理导致Eb和ESb肿瘤细胞系上I类组织相容性抗原表达增强。在ESb细胞中，IFN-γ处理后Ia和与Ia相关的恒定链（Ii）的表达也增加。对Eb细胞系进行IFN-γ处理后，未观察到Ia和Ii抗原的诱导。这些研究表明，Eb和ESb肿瘤细胞系在以下方面存在显著差异：（i）II类主要组织相容性抗原的组成性表达，以及（ii）对IFN-γ处理的反应。
