Yamauchi H, Kakizaki K, Maeda M, Fukaya Y, Nakamura R, Miyagawa K
Tohoku J Exp Med. 1985 Dec;147(4):379-87. doi: 10.1620/tjem.147.379.
Ligation and section of the main and accessory pancreatic ducts were performed in adult mongrel dogs. Four weeks after the procedure we carried out total pancreatectomy in six duct-ligated dogs, and an attempt was made to prepare the graft of islets from the highly scarred pancreas of the duct-ligated dogs by means of a newly developed collagenase perfusion method. We succeeded to normalize the high fasting glucose level which occurred after the total pancreatectomy in three of six dogs through the autotransplantation of the procured graft. However, insulin secretion was severely depressed in transplanted animals. Two of the three dogs showed high plasma glucose levels 15 and 20 weeks after the transplantation, respectively. Histological examination of the spleen revealed well preserved islets with good immunocytochemical insulin staining in one transplanted dog whose plasma glucose level was controlled for about five months. No pancreatic exocrine tissue was detected in the histological sections of the spleen in the transplanted dogs and in a transplanted control dog.
在成年杂种犬身上进行了主胰管和副胰管的结扎及切断术。术后四周，我们对六只结扎导管的犬实施了全胰切除术，并尝试通过一种新开发的胶原酶灌注方法，从结扎导管犬高度瘢痕化的胰腺中制备胰岛移植物。通过移植所获取的移植物，我们成功使六只犬中的三只在全胰切除术后出现的高空腹血糖水平恢复正常。然而，移植动物的胰岛素分泌严重受抑。三只犬中的两只分别在移植后15周和20周出现高血糖水平。对一只移植犬的脾脏进行组织学检查，其血糖水平在约五个月内得到控制，结果显示胰岛保存良好，免疫细胞化学胰岛素染色良好。在移植犬和一只移植对照犬的脾脏组织切片中未检测到胰腺外分泌组织。
