Schottdorf Manuel, Rich P Dylan, Diamanti E Mika, Lin Albert, Tafazoli Sina, Nieh Edward H, Thiberge Stephan Y
Princeton Neuroscience Institute, Princeton University, Princeton, NJ, USA.
Psychological and Brain Sciences, University of Delaware, Newark, DE, USA.
bioRxiv. 2025 Jan 12:2024.09.23.612766. doi: 10.1101/2024.09.23.612766.
Many laboratories use two-photon microscopy through commercial suppliers, or homemade designs of considerable complexity. The integrated nature of these systems complicates customization, troubleshooting, and training on the principles of two-photon microscopy. Here, we present "Twinkle": a microscope for Two-photon Imaging in Neuroscience, and Kit for Learning and Education. It is a fully open, high performing and easy-to-set-up microscope that can effectively be used for both education and research. The instrument features a > 1 mm field of view, using a modern objective with 3 mm working distance and 2 inch diameter optics combined with GaAsP photomultiplier tubes to maximize the fluorescence signal. We document our experiences using this system as a teaching tool in several two week long workshops, exemplify scientific use cases, and conclude with a broader note on the place of our work in the growing space of open scientific instrumentation.
许多实验室通过商业供应商或自行设计的复杂程度相当高的自制设备来使用双光子显微镜。这些系统的集成特性使得定制、故障排除以及双光子显微镜原理的培训变得复杂。在此，我们展示“Twinkle”：一款用于神经科学双光子成像的显微镜以及学习与教育套件。它是一款完全开放、高性能且易于搭建的显微镜，可有效地用于教育和研究。该仪器具有大于1毫米的视野，采用具有3毫米工作距离和2英寸直径光学元件的现代物镜，并结合砷化镓磷光电倍增管以最大化荧光信号。我们记录了在多个为期两周的工作坊中使用该系统作为教学工具的经验，举例说明了科学应用案例，并在开放科学仪器不断发展的背景下，对我们工作的地位进行了更广泛的阐述。
