Tetz George, Kardava Kristina, Vecherkovskaya Maria, Khodadadi-Jamayran Alireza, Tsirigos Aristotelis, Tetz Victor
bioRxiv. 2024 Sep 25:2024.09.11.612522. doi: 10.1101/2024.09.11.612522.
Our previous studies revealed the existence of a Universal Receptive System that regulates interactions between cells and their environment. This system is composed of DNA- and RNA-based Teazeled receptors (TezRs) found on the surface of prokaryotic and eukaryotic cells, as well as integrases and recombinases.. In the current study, we aimed to provide further insight into the regulatory role of TezR and its loss in gene transcription. To this end, transcriptomic analysis of MSSA VT209 was performed following the destruction of TezRs. Bacterial RNA samples were extracted from nuclease-treated and untreated MSSA VT209. After destruction of the DNA-based-, RNA-, or combined DNA- and RNA-based TezRs of , 103, 150, and 93 genes were significantly differently expressed, respectively. The analysis revealed differential clustering of gene expression following the loss of different TezRs, highlighting individual cellular responses following the loss of DNA- and RNA-based TezRs. KEGG pathway gene enrichment analysis revealed that the most upregulated pathways following TezR inactivation included those related to energy metabolism, cell wall metabolism, and secretion systems. Some of the genetic pathways were related to the inhibition of biofilm formation and increased antibiotic resistance, and we confirmed this at the phenotypic level using studies. The results of this study add another line of evidence that the Universal Receptive System plays an important role in cell regulation, including cell responses to the environmental factors of clinically important pathogens, and that nucleic acid-based TezRs are functionally active parts of the extrabiome.
我们之前的研究揭示了一种普遍接受系统的存在，该系统调节细胞与其环境之间的相互作用。这个系统由原核细胞和真核细胞表面发现的基于DNA和RNA的刺果受体（TezRs）以及整合酶和重组酶组成。在当前的研究中，我们旨在进一步深入了解TezR的调节作用及其在基因转录中的缺失情况。为此，在破坏TezRs后对MSSA VT209进行了转录组分析。从经核酸酶处理和未经处理的MSSA VT209中提取细菌RNA样本。在分别破坏基于DNA、RNA或基于DNA和RNA组合的TezRs后，分别有103、150和93个基因显著差异表达。分析揭示了不同TezRs缺失后基因表达的差异聚类，突出了基于DNA和RNA的TezRs缺失后的个体细胞反应。KEGG通路基因富集分析表明，TezR失活后上调最多的通路包括与能量代谢、细胞壁代谢和分泌系统相关的通路。一些遗传通路与生物膜形成的抑制和抗生素耐药性增加有关，我们在表型水平上通过研究证实了这一点。这项研究的结果增加了另一项证据，即普遍接受系统在细胞调节中发挥重要作用，包括细胞对临床重要病原体环境因素的反应，并且基于核酸的TezRs是细胞外组的功能活性部分。
