Gómez Fernando, Navarrete-Carlos Tania Corina, López-Osorio Yahir Enrique, Zhang Huan, Raymond Eugenio, Salas Rafael, Alonso-Rodríguez Rosalba, Lin Senjie
Carmen Campos Panisse 3, Puerto de Santa Maria, Spain.
Unidad Académica Mazatlán, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Mazatlan, Sinaloa, Mexico.
J Eukaryot Microbiol. 2024 Nov-Dec;71(6):e13062. doi: 10.1111/jeu.13062. Epub 2024 Oct 10.
The planktonic dinoflagellate genus Centrodinium has been understudied, with the type species C. elongatum remaining undocumented since the original description. Here, we report C. elongatum isolated from Mazatlán, Mexican Pacific. In the chains, the posterior daughter cell with an incomplete apical horn shows the morphology of C. elongatum, while the anterior daughter cell with complete epitheca corresponds to C. pulchrum. For the first time, a species of Centrodinium sensu stricto (highly laterally flattened species with horns) was cultured. An unarmored life stage, known as Murrayella ovalis, derived from the spheroplast after ecdysis. In the rDNA molecular phylogenies, C. elongatum (=C. pulchrum) nested as basal to morphologically similar species (C. eminens and C. intermedium) and as a sister group of a former Murrayella species, C. punctatum. C. elongatum differs from C. eminens and C. intermedium in the chain formation, second apical (2') plate not being divided, horns with coarse poroid ornamentation, and missing prominent distal spinules. The taxonomy of Centrodinium sensu stricto is revised, with a discussion in the identities of C. complanatum, C. eminens, and C. maximum. The name C. deflexum is restored as a senior synonym of C. intermedium and C. ovale.
浮游性甲藻Centrodinium属一直未得到充分研究，自最初描述以来，其模式种细长Centrodinium elongatum仍无文献记载。在此，我们报告从墨西哥太平洋沿岸的马萨特兰分离出的细长Centrodinium elongatum。在链中，具有不完全顶角的后仔细胞呈现出细长Centrodinium elongatum的形态，而具有完整上壳的前仔细胞则对应于美丽Centrodinium pulchrum。首次培养了一种狭义的Centrodinium属物种（具有角的高度侧向扁平物种）。一种无甲的生活阶段，即卵形穆雷藻（Murrayella ovalis），是由脱壳后的球状体衍生而来。在rDNA分子系统发育中，细长Centrodinium elongatum（=美丽Centrodinium pulchrum）嵌套在形态相似的物种（突出Centrodinium eminens和中间Centrodinium intermedium）的基部，并且是前穆雷藻属物种点状Centrodinium punctatum的姐妹群。细长Centrodinium elongatum在链的形成、第二顶（2'）板不分裂、角上有粗糙的孔状纹饰以及缺少突出的远端小刺方面与突出Centrodinium eminens和中间Centrodinium intermedium不同。对狭义Centrodinium属的分类进行了修订，并讨论了扁平Centrodinium complanatum、突出Centrodinium eminens和大Centrodinium maximum的身份。恢复了弯曲Centrodinium deflexum这个名称，作为中间Centrodinium intermedium和卵形Centrodinium ovale的 senior同义词。
