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Quantitative real-time in vitro transcription assay (QRIVTA) for transcriptional regulation studies.

作者信息

Liu Fan, Xu Jing, Hu Xinli, Duan Bo, Xia Bin

机构信息

School of Life Sciences, Peking University, Beijing 100871, China.

Beijing Nuclear Magnetic Resonance Center, Peking University, Beijing 100871, China.

出版信息

Protein Cell. 2025 Jun 20;16(6):484-490. doi: 10.1093/procel/pwae054.

DOI:10.1093/procel/pwae054
PMID:39387643
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12187118/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c551/12187118/b3d3fa3d1f9c/pwae054_fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c551/12187118/1e63456dca03/pwae054_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c551/12187118/b3d3fa3d1f9c/pwae054_fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c551/12187118/1e63456dca03/pwae054_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c551/12187118/b3d3fa3d1f9c/pwae054_fig2.jpg

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Nucleic Acids Res. 2024 Mar 21;52(5):2157-2173. doi: 10.1093/nar/gkae090.
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High-throughput, fluorescent-aptamer-based measurements of steady-state transcription rates for the Mycobacterium tuberculosis RNA polymerase.
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Nucleic Acids Res. 2023 Oct 27;51(19):e99. doi: 10.1093/nar/gkad761.
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Fluorogenic RNA aptamers to probe transcription initiation and co-transcriptional RNA folding by multi-subunit RNA polymerases.荧光 RNA 适体用于探测多亚基 RNA 聚合酶的转录起始和共转录 RNA 折叠。
Methods Enzymol. 2022;675:207-233. doi: 10.1016/bs.mie.2022.07.010. Epub 2022 Aug 19.
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Target-triggered transcription machinery for ultra-selective and sensitive fluorescence detection of nucleoside triphosphates in one minute.一分钟内超选择性和灵敏性检测核苷三磷酸的靶触发转录机制。
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