Allen G, Paynter C A, Winther M D
J Cell Sci Suppl. 1985;3:29-38. doi: 10.1242/jcs.1985.supplement_3.4.
Mouse epidermal growth factor (EGF) is under investigation as a deflecting agent for sheep. Substantial quantities of the pure protein are required for these studies and to supply this need a gene for the protein was synthesized and inserted into plasmid vectors to direct the expression of EGF polypeptide, or fusion proteins containing the EGF peptide sequence, in transformed Escherichia coli. Mature EGF was released by lysine specific proteolysis of a fusion protein consisting of part of the E. coli TrpE protein, a lysine linker and EGF polypeptide. The EGF was purified and characterized and was found to be biologically active.
小鼠表皮生长因子（EGF）正作为绵羊的一种偏转剂进行研究。这些研究需要大量的纯蛋白，为满足这一需求，合成了该蛋白的基因，并将其插入质粒载体中，以指导EGF多肽或包含EGF肽序列的融合蛋白在转化的大肠杆菌中表达。成熟的EGF通过对由大肠杆菌TrpE蛋白的一部分、一个赖氨酸连接子和EGF多肽组成的融合蛋白进行赖氨酸特异性蛋白酶解而释放出来。对EGF进行了纯化和表征，发现其具有生物活性。
