College of Animal Science, Anhui Science and Technology University, Fengyang, China.
Anim Sci J. 2024 Jan-Dec;95(1):e14005. doi: 10.1111/asj.14005.
Ochratoxin A (OTA) widely exists in raw food and feed materials and can induce liver damage and toxicity. However, the mechanisms of OTA-induced hepatotoxicity were largely unknown. Thus, our study aimed to uncover the vital genes relevant to OTA-induced hepatotoxicity in broiler chickens. Gene expression data of chicken embryo primary hepatocytes (CEPHs) in OTA-treated and control groups were obtained from the GEO database. Totally 1407 differentially expressed genes (DEGs) were selected, of which 850 and 557 genes were up- and downregulated in OTA-treated CEPHs. Gene ontology (GO) enrichment revealed that the DEGs were in connection with various biological processes, such as signal transduction, extracellular matrix organization, axon guidance, cell division, cholesterol homeostasis, proteolysis, microtubule cytoskeleton organization, and chromosome segregation. Pathway enrichment showed that the DEGs were related to metabolic pathways, ferroptosis, calcium, FoxO, Wnt, cell cycle, apoptosis, calcium, and cell adhesion molecules signaling pathways. Furthermore, the hub genes, including CDK1, DLGAP5, KIF2C, VCL, ITGB3, and ZYX, were identified as hub genes potentially contributing to OTA-induced hepatotoxicity. Taken together, this study provides valuable insights into the mechanisms underlying OTA-induced hepatotoxicity in broiler chickens.
赭曲霉毒素 A（OTA）广泛存在于生食品和饲料原料中，可引起肝损伤和毒性。然而，OTA 诱导肝毒性的机制在很大程度上尚不清楚。因此，我们的研究旨在揭示与肉鸡中 OTA 诱导肝毒性相关的重要基因。从 GEO 数据库中获得了 OTA 处理和对照组鸡胚原代肝细胞（CEPHs）的基因表达数据。总共选择了 1407 个差异表达基因（DEGs），其中 850 个和 557 个基因在 OTA 处理的 CEPHs 中上调和下调。基因本体（GO）富集显示，DEGs 与各种生物学过程有关，如信号转导、细胞外基质组织、轴突导向、细胞分裂、胆固醇稳态、蛋白水解、微管细胞骨架组织和染色体分离。途径富集表明，DEGs 与代谢途径、铁死亡、钙、FoxO、Wnt、细胞周期、细胞凋亡、钙和细胞黏附分子信号通路有关。此外，鉴定出包括 CDK1、DLGAP5、KIF2C、VCL、ITGB3 和 ZYX 在内的枢纽基因，这些基因可能是导致 OTA 诱导肝毒性的枢纽基因。总之，这项研究为了解肉鸡中 OTA 诱导肝毒性的机制提供了有价值的见解。
