Department of Biology, Kyung Hee University, Seoul, Republic of Korea.
Department of Gastroenterology, Center for Crohn's and Colitis, Kyung Hee University Hospital, Kyung Hee University College of Medicine, Seoul, Republic of Korea.
Sci Rep. 2024 Oct 10;14(1):23701. doi: 10.1038/s41598-024-74002-6.
Fecal samples were collected from 640 individuals in Korea, including 523 patients with IBD (223 with Crohn's disease [CD] and 300 with ulcerative colitis [UC]) and 117 healthy controls. The samples were subjected to cross-sectional gut metagenomic analysis using 16 S rRNA sequencing and bioinformatics analysis. Patients with IBD, particularly those with CD, exhibited significantly lower alpha diversities than the healthy subjects. Differential abundance analysis revealed dysbiotic signatures, characterized by an expansion of the genus Escherichia-Shigella in patients with CD. Functional annotations showed that functional pathways related to bacterial pathogenesis and production of hydrogen sulfide (HS) were strongly upregulated in patients with CD. A dysbiosis score, calculated based on functional characteristics, highly correlated with disease severity. Markers distinguishing between healthy subjects and patients with IBD showed accurate classification based on a small number of microbial taxa, which may be used to diagnose ambiguous cases. These findings confirm the taxonomic and functional dysbiosis of the gut microbiota in patients with IBD, especially those with CD. Taxa indicative of dysbiosis may have significant implications for future clinical research on the management and diagnosis of IBD.
从韩国的 640 个人中收集了粪便样本，包括 523 名 IBD 患者（223 名克罗恩病 [CD] 患者和 300 名溃疡性结肠炎 [UC] 患者）和 117 名健康对照者。使用 16S rRNA 测序和生物信息学分析对这些样本进行了横断面肠道宏基因组分析。IBD 患者，特别是 CD 患者，其α多样性明显低于健康受试者。差异丰度分析显示出了一种生态失调的特征，即 CD 患者的大肠杆菌-志贺氏菌属扩张。功能注释显示，与细菌发病机制和硫化氢（HS）产生相关的功能途径在 CD 患者中强烈上调。基于功能特征计算的失调评分与疾病严重程度高度相关。能够区分健康受试者和 IBD 患者的标志物能够基于少数微生物类群进行准确的分类，这可能用于诊断不明确的病例。这些发现证实了 IBD 患者，特别是 CD 患者的肠道微生物群的分类和功能失调。提示生态失调的分类群可能对未来 IBD 的管理和诊断的临床研究具有重要意义。
