Department of Bioscience Engineering, University of Antwerp, Groenenborgerlaan 171, Antwerpen, B-2020, Belgium.
YUN NV, Galileilaan 15, Niel, B-2845, Belgium.
Sci Rep. 2024 Oct 10;14(1):23659. doi: 10.1038/s41598-024-74513-2.
Atopic dermatitis is the most common inflammatory skin condition with a severe negative impact on patients' quality of life. The etiology of AD is complex and depends on age, genetics, the immune system, environmental factors, and the skin microbiome, with a key role for pathogenic Staphylococcus aureus in the development of severe AD. However, the composition of the skin microbiome in mild AD is understudied. Here, using metagenomic shallow shotgun sequencing, we showed that mild AD lesions did not show a significant difference in the diversity of the skin microbiome compared to samples from non-AD patients and that the relative abundance of S. aureus did not differ in these mild AD lesions. However, when we assessed other taxa, Mycobacterium ostraviense, Pedobacter panaciterrae_A and four Streptomyces species were identified with higher abundances in mild AD lesions and species of 15 genera were decreased in abundance. The highest fold decreases were observed for Paracoccus marcusii, Microbacterium lacticum, Micrococcus luteus, and Moraxella sp002478835. These microbiome compositional insights are a first step towards novel microbiome-based diagnostics and therapeutics for early intervention at the stage of mild AD and provide a path forward for the functional study of species involved in this often-overlooked patient population.
特应性皮炎是最常见的炎症性皮肤疾病，对患者的生活质量有严重的负面影响。AD 的病因复杂，取决于年龄、遗传、免疫系统、环境因素和皮肤微生物组，其中致病性金黄色葡萄球菌在严重 AD 的发展中起着关键作用。然而，轻度 AD 皮肤微生物组的组成仍研究不足。在这里，我们使用宏基因组浅层 shotgun 测序表明，与非 AD 患者的样本相比，轻度 AD 病变的皮肤微生物组多样性没有显著差异，这些轻度 AD 病变中金黄色葡萄球菌的相对丰度也没有差异。然而，当我们评估其他分类群时，发现嗜麦芽寡养单胞菌、泛生枝动杆菌 A 和四种链霉菌物种在轻度 AD 病变中丰度较高，15 个属的物种丰度降低。丰度下降幅度最大的是马库斯副球菌、乳微球菌、黄色微球菌和莫拉氏菌 sp002478835。这些微生物组组成的见解是朝着基于微生物组的早期干预轻度 AD 阶段的新型诊断和治疗方法迈出的第一步，并为参与这一经常被忽视的患者群体的物种的功能研究提供了前进的道路。
