Faculty of Dentistry, Federal University of Alagoas, Maceió, Brazil.
Institute of Pharmaceutical Sciences, Federal University of Alagoas, Maceió, Brazil.
Eur J Oral Sci. 2024 Dec;132(6):e13023. doi: 10.1111/eos.13023. Epub 2024 Oct 10.
Water treeing and water droplets are observed within adhesive layers and on the hybridized surface after bonding sound dentin using single-bottle etch-and-rinse adhesives, indicating permeability of the hybrid layer to water. The aim of this study was to assess the efficacy of dentin sealing by adhesives containing propolis by quantifying the area of water transudation from dentinal tubules after dentin hybridization. Brazilian red propolis was added to experimental adhesive and Single Bond (3M/ESPE) adhesive; experimental adhesive and Single Bond without propolis were used as controls. Under simulated pulp pressure, two layers of adhesive were applied to etched human dentin discs. Three minutes after light-curing, the hybridized dentin surface was replicated, and epoxy resin replicas were created to obtain scanning electron microscope images. Data were evaluated using ANOVA and Tukey's test. Single Bond containing propolis significantly decreased water permeation through the hybrid layer compared with the control group. Three minutes after polymerization, the experimental adhesive without propolis had formed a permeable hybrid layer. The addition of Brazilian red propolis significantly reduced surface water on hybridized dentin in a concentration-dependent manner. Two-step etch-and-rinse adhesives containing propolis were effective in reducing water permeation through the hybridized dentin surface.
采用单瓶酸蚀-冲洗型黏结剂黏结完整牙本质后，可在黏结层内及杂交界面观察到水树和液滴，这表明混合层对水具有通透性。本研究旨在通过定量分析牙本质混合后牙本质小管内水分渗出的面积来评估含蜂胶黏结剂对牙本质封闭的效果。将巴西红蜂胶添加到实验性黏结剂和单组分黏结剂（3M/ESPE）中；实验组和不含蜂胶的单组分黏结剂作为对照。在模拟牙髓压力下，将两层黏结剂应用于酸蚀后的人牙本质片上。光固化 3 分钟后，复制杂交牙本质表面，并制作环氧树脂复制品以获取扫描电子显微镜图像。使用方差分析和 Tukey 检验对数据进行评估。与对照组相比，含蜂胶的单组分黏结剂显著降低了混合层中的水分渗透。光固化 3 分钟后，不含蜂胶的实验性黏结剂形成了可渗透的混合层。巴西红蜂胶的添加以浓度依赖的方式显著减少了杂交牙本质表面的水分。含蜂胶的两步酸蚀-冲洗黏结剂可有效降低混合牙本质表面的水分渗透。
