Molnár J, Prágai B, Berencsi K, Mándi Y, Földeák S
Acta Microbiol Hung. 1985;32(4):379-86.
The effects of rutin-N-mustard, amantadine-N-mustard, chlorpromazine and human interferon types alpha, beta and gamma (IFN-alpha, -beta and -gamma) were studied on the DNA, RNA and protein synthesis of K-562 cells. Monocyte-mediated cytotoxicity and immune spleen cell activity were examined in the presence of the same compounds (except for IFN-beta). The natural killer (NK) cell activity was tested in the presence of the two chlorpromazine compounds and the two N-mustard derivatives. Only 7,8-dioxochlorpromazine exerted an inhibitory effect on DNA synthesis. The protein synthesis of the cells was inhibited in the presence of IFN-alpha, -beta and -gamma. 7,8-Dioxochlorpromazine exerted some inhibition on both NK and immune spleen cell activity, while monocyte-mediated cytolysis was not altered. IFN-alpha, -beta and -gamma activated the cytolytic activity of monocytes and the NK activity in control experiments. Chlorpromazine, rutin-N-mustard and amantadine-N-mustard were ineffective in both tests in vitro. Rutin-N-mustard, 7,8-dioxochlorpromazine and the interferons may be assumed to have quite different antiproliferative mechanisms of actions.
研究了芦丁 - N - 芥子气、金刚烷胺 - N - 芥子气、氯丙嗪以及人α、β、γ型干扰素（IFN -α、-β和 -γ）对K - 562细胞DNA、RNA和蛋白质合成的影响。在存在相同化合物（除IFN -β外）的情况下检测了单核细胞介导的细胞毒性和免疫脾细胞活性。在存在两种氯丙嗪化合物和两种N - 芥子气衍生物的情况下测试了自然杀伤（NK）细胞活性。只有7,8 - 二氧氯丙嗪对DNA合成有抑制作用。在IFN -α、-β和 -γ存在的情况下细胞的蛋白质合成受到抑制。7,8 - 二氧氯丙嗪对NK和免疫脾细胞活性都有一定抑制作用，而单核细胞介导的细胞溶解作用未改变。在对照实验中，IFN -α、-β和 -γ激活了单核细胞的细胞溶解活性和NK活性。氯丙嗪、芦丁 - N - 芥子气和金刚烷胺 - N - 芥子气在两种体外试验中均无效。芦丁 - N - 芥子气、7,8 - 二氧氯丙嗪和干扰素可能具有完全不同的抗增殖作用机制。
