Hossian Shajjad, Reza Md Faijan, Rahman Mohammad Matiur, Alam Mohammed Jahangir, Razzak Md Abdur, Mollah Md Fazlul Awal, Tiersch Terrence R, Sarder Md Rafiqul Islam
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Aquatic Germplasm and Genetic Resources Center, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA.
Aquac Res. 2024 Jan;2024(1). doi: 10.1155/2024/9655558. Epub 2024 Oct 1.
Grass carp (), a freshwater nonnative fish species, is a potential aquaculture candidate in Bangladesh. The seed of the species is produced in the hatcheries by hypophysation, but the quality of seedstock of grass carp is deteriorated due to inbreeding, negative selection, and interspecific introgression among fishes. To increase the availability of quality seed and best genetic traits of grass carp, this study dealt with finding suitable conditions of sperm cryopreservation protocols and evaluated the effectiveness of cryopreserved sperm through breeding trials of . A broodstock population was developed from fingerlings imported from China by the Bangladesh Department of Fisheries. Sperm was collected from hormone-induced mature males, with an estimated concentration of 2.4 ± 0.3 × 10 cells/mL and a pH of 8.3 ± 0.2. Sperm motility was evaluated in different concentrations of NaCl solution. The highest motility (96% ± 1%) and longest motility duration (22 ± 1 min) were achieved at 0.4% of NaCl (128 mOsmol/kg). The toxicity of DMSO and methanol at concentrations of 5%, 10%, and 15% was evaluated for 5-40 min using Alsever's solution and egg yolk citrate. The highest motility was achieved during 5 and 10 min of incubation with cryoprotectants at 5% and 10%. Alsever's solution with 10% DMSO at 1:9 dilution with sperm produced the highest equilibration motility (93% ± 2%) and when cooled at 10°C/min yielded the highest postthaw motility (85% ± 3%). Fertilization of 24% ± 3% to 51% ± 2% and hatching of 18% ± 2% to 41% ± 2% were achieved by using cryopreserved sperm in six selected private hatcheries. The fertilization rate for fresh sperm sourced from hatchery-reared males was 64% ± 5% to 85% ± 3%, and the hatching rate ranged from 53% ± 6% to 74% ± 5%. Thus, the cryopreservation protocol of sperm was found to be feasible for fry production at commercial hatcheries, but further research is needed to improve the fertilization and hatching rates.
草鱼是一种非本地淡水鱼类，是孟加拉国水产养殖的潜在候选品种。该物种的鱼苗在孵化场通过垂体激素催产法生产，但由于近亲繁殖、负选择以及鱼类间的种间基因渗入，草鱼亲鱼的质量有所下降。为了提高优质鱼苗的可得性以及草鱼的最佳遗传特性，本研究致力于寻找精子冷冻保存方案的合适条件，并通过……的繁殖试验评估冷冻精子的有效性。孟加拉国渔业部从中国进口的鱼种培育出了亲鱼群体。从激素诱导成熟的雄性草鱼采集精子，估计精子浓度为2.4±0.3×10⁹个细胞/毫升，pH值为8.3±0.2。在不同浓度的氯化钠溶液中评估精子活力。在0.4%的氯化钠（128毫摩尔/千克）溶液中精子活力最高（96%±1%），活力持续时间最长（22±1分钟）。使用阿氏液和蛋黄柠檬酸盐评估了浓度为5%、10%和15%的二甲基亚砜（DMSO）和甲醇在5至40分钟内的毒性。在与5%和10%的冷冻保护剂孵育5分钟和10分钟时精子活力最高。用1:9稀释的含10%DMSO的阿氏液与精子混合，平衡后活力最高（93%±2%），以10℃/分钟的速度冷却时解冻后活力最高（85%±3%）。在六个选定的私人孵化场使用冷冻精子实现了24%±3%至 51%±2%的受精率和18%±2%至41%±2%的孵化率。来自孵化场养殖雄性草鱼的新鲜精子的受精率为64%±5%至85%±3%，孵化率为53%±6%至74%±5%。因此，发现草鱼精子的冷冻保存方案对于商业孵化场的鱼苗生产是可行的，但需要进一步研究以提高受精率和孵化率。
