血细胞计数对照品与新鲜患者乙二胺四乙酸抗凝血液标本的流变学行为比较。

Comparison of the rheological behavior of hemocytometry controls and fresh patient EDTA-anticoagulated blood specimens.

作者信息

Lombarts A J, Goslinga H, Heuvelmans J H, Leijnse B

出版信息

Haematologia (Budap). 1985;18(4):249-58.

Abstract

For rheological reasons high viscosity specimens are more sensitive to deficiencies, if any, in the aspiration systems of whole blood flow cytometers and consequently are more likely to give erroneous values. For this reason the rheological behavior of hemocytometry controls was compared with that of patient blood specimens. It is shown that the behavior of controls and patient specimens of comparable hematocrit is similar at shear rates that are probably occurring during aspiration (exceeding approximately 40 s-1). The hematocrit is shown to be a major determinant of the viscosity of both controls and patient specimens. The results suggest that from the rheological point of view the fundamental IFCC premise is met, "that errors detected by means of control specimens exactly mirror errors occurring with patients' specimens", provided their hematocrits are taken into account.

摘要

由于流变学原因，高粘度标本对全血流式细胞仪抽吸系统中的缺陷（如果有的话）更为敏感，因此更有可能给出错误值。出于这个原因，将血细胞计数质控品的流变学行为与患者血液标本的流变学行为进行了比较。结果表明，在抽吸过程中可能出现的剪切速率（超过约40 s-1）下，具有可比血细胞比容的质控品和患者标本的行为相似。血细胞比容被证明是质控品和患者标本粘度的主要决定因素。结果表明，从流变学角度来看，国际临床化学和检验医学联合会（IFCC）的基本前提得到了满足，即“通过质控品检测到的误差准确反映了患者标本中出现的误差”，前提是考虑到它们的血细胞比容。