Scorzo Augustino V, Kwon Caleb Y, Strawbridge Rendall R, Duke Ryan B, Warner William R, Chen Kristen L, Li Chengpei, Fan Xiaoyao, Roberts David W, Paulsen Keith D, Davis Scott C
Dartmouth College, Thayer School of Engineering.
Dartmouth Hitchcock Medical Center, Neurosurgery.
Proc SPIE Int Soc Opt Eng. 2024 Jan-Feb;12825. doi: 10.1117/12.3003221. Epub 2024 Mar 12.
Fluorescence cryo-imaging is a high-resolution optical imaging technique that produces 3-D whole-body biodistributions of fluorescent molecules within an animal specimen. To accomplish this, animal specimens are administered a fluorescent molecule or reporter and are frozen to be autonomously sectioned and imaged at a temperature of -20°C or below. Thus, to apply this technique effectively, administered fluorescent molecules should be relatively invariant to low temperature conditions for cryo-imaging and ideally the fluorescence intensity should be stable and consistent in both physiological and cryo-imaging conditions. Herein, we assessed the mean fluorescence intensity of 11 fluorescent contrast agents as they are frozen in a tissue-simulating phantom experiment and show an example of a tested fluorescent contrast agent in a cryo-imaged whole pig brain. Most fluorescent contrast agents were stable within ~25% except for FITC and PEGylated FITC derivatives, which showed a dramatic decrease in fluorescence intensity when frozen.
荧光冷冻成像技术是一种高分辨率光学成像技术，可生成动物标本内荧光分子的三维全身生物分布。为此，给动物标本注射一种荧光分子或报告物，然后将其冷冻，以便在-20°C或更低温度下自动切片并成像。因此，为了有效应用该技术，所注射的荧光分子在冷冻成像的低温条件下应相对稳定，理想情况下，荧光强度在生理和冷冻成像条件下都应稳定且一致。在此，我们在组织模拟体模实验中评估了11种荧光造影剂冷冻时的平均荧光强度，并展示了在冷冻成像的全猪脑中测试的一种荧光造影剂的示例。除了FITC和聚乙二醇化FITC衍生物外，大多数荧光造影剂在约25%的范围内是稳定的，FITC和聚乙二醇化FITC衍生物在冷冻时荧光强度显著降低。
