Haun Brien K, To Albert, Williams Caitlin A, Ball Aquena, Fong Karalyn, Wong Teri Ann S, Shobayo Bode, Teahton Julius, Ching Lauren, Kamara Varney, Tekah Davidetta M, Humphrey Peter, Berestecky John, Nerurkar Vivek R, Lehrer Axel T
Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96813, USA.
Department of Tropical Medicine, Medical Microbiology and Pharmacology, John A Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96813, USA.
Immuno. 2024 Mar;4(1):108-124. doi: 10.3390/immuno4010007. Epub 2024 Mar 3.
The SARS-CoV-2 pandemic ignited global efforts to rapidly develop testing, therapeutics, and vaccines. However, the rewards of these efforts were slow to reach many low- to middle-income countries (LMIC) across the African continent and globally. Therefore, two bead-based multiplexed serological assays were developed to determine SARS-CoV-2 exposure across four counties in Liberia. This study was conducted during the summer of 2021 on 189 samples collected throughout Grand Bassa, Bong, Margibi, and Montserrado counties. Our multiplexed immunoassay (MIA) detected elevated exposure to SARS-CoV-2 and multiple variant antigens. Additionally, we detected evidence of exposure to Dengue virus serotype 2, Chikungunya virus, and the seasonal coronavirus NL63. Our multiplexed inhibition test (MINT) was developed from the MIA to observe antibody-mediated inhibition of SARS-CoV-2 spike protein binding to its cognate cellular receptor ACE-2. We detected inhibitory antibodies in the tested Liberian samples, which were collectively consistent with a convalescent serological profile. These complementary assays serve to supplement existing serological testing needs and may enhance the technical capacity of scientifically underrepresented regions globally.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）大流行引发了全球迅速开发检测方法、治疗药物和疫苗的努力。然而，这些努力的成果在非洲大陆乃至全球的许多低收入和中等收入国家（LMIC）却姗姗来迟。因此，开发了两种基于微珠的多重血清学检测方法，以确定利比里亚四个县的SARS-CoV-2感染情况。本研究于2021年夏季对大巴萨、邦、马吉比和蒙特塞拉多四个县收集的189份样本进行。我们的多重免疫分析（MIA）检测到SARS-CoV-2和多种变异抗原的暴露水平升高。此外，我们还检测到了感染登革热病毒2型、基孔肯雅病毒和季节性冠状病毒NL63的证据。我们的多重抑制试验（MINT）是在MIA的基础上开发的，用于观察抗体介导的对SARS-CoV-2刺突蛋白与其同源细胞受体血管紧张素转换酶2（ACE-2）结合的抑制作用。我们在测试的利比里亚样本中检测到了抑制性抗体，这些抗体总体上与恢复期血清学特征一致。这些互补检测方法有助于满足现有的血清学检测需求，并可能提高全球科学研究较少地区的技术能力。
