Department of Biotechnology, University of Ribeirão Preto, Ribeirão Preto São Paulo, Brazil.
Department of Biotechnology, University of Ribeirão Preto, Ribeirão Preto São Paulo, Brazil; Medical School, University of Ribeirão Preto, Ribeirão Preto São Paulo, Brazil.
Acta Neurobiol Exp (Wars). 2024 Oct 11;84(3):266-274. doi: 10.55782/ane-2024-2575.
Evidence is provided that the glycosylated flavonoid vitexin (apigenin‑8‑C‑beta‑D‑glucopyranoside) attenuates pentylenetetrazole (PTZ)‑induced acute tonic‑clonic seizures in rats. However, the effects of chronic and systemic vitexin in PTZ‑kindled rats remain unknown. The aim of this work was to investigate the effect of long‑term treatment with vitexin in the PTZ‑kindling model of epilepsy. Male Wistar rats received intraperitoneal injections of PTZ at a subconvulsive dose of 35 mg/kg every other day for 29 days. Either saline containing dimethyl sulfoxide - DMSO 1% (vehicle), diazepam (2 mg/kg; positive control) or vitexin (2.5 mg/kg) was administered intraperitoneally 30 min before each PTZ injection. The behavioral reactions were recorded by 30 min immediately after each PTZ injection. Furthermore, on the 31st day, that is, 48 h after the latter dose of PTZ, the animals were euthanized and renal and hepatic biochemical markers were evaluated in blood serum. Chronic treatment with either diazepam or vitexin attenuated the seizures provoked by PTZ injections. Neither diazepam nor vitexin caused changes in renal levels of creatinine and urea and in hepatic levels of aspartate aminotransferase and alanine aminotransferase. Our findings suggest that chronic administration of vitexin attenuates the progression of PTZ‑induced kindling without causing side effects on kidneys and liver.
有证据表明，糖基化黄酮类化合物牡荆素（芹菜素-8-C-β-D-吡喃葡萄糖苷）可减轻戊四氮（PTZ）诱导的大鼠强直阵挛性癫痫发作。然而，慢性和全身性牡荆素在 PTZ 点燃大鼠中的作用尚不清楚。本工作旨在研究长期给予牡荆素对癫痫 PTZ 点燃模型的影响。雄性 Wistar 大鼠每隔一天接受腹腔注射 35mg/kg 的 PTZ 亚惊厥剂量，共 29 天。在每次 PTZ 注射前 30 分钟，给予含有 1%二甲基亚砜（DMSO）的生理盐水（载体）、地西泮（2mg/kg；阳性对照）或牡荆素（2.5mg/kg）。通过立即在每次 PTZ 注射后 30 分钟记录行为反应。此外，在第 31 天，即在最后一次 PTZ 剂量后 48 小时，处死动物并评估血清中的肾和肝生化标志物。慢性给予地西泮或牡荆素均可减轻 PTZ 注射引起的癫痫发作。地西泮和牡荆素均未引起血清肌酐和尿素氮以及天门冬氨酸氨基转移酶和丙氨酸氨基转移酶肝水平的变化。我们的发现表明，慢性给予牡荆素可减轻 PTZ 诱导的点燃进展，而不会对肾脏和肝脏造成副作用。
