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使用标准化行为分析方法研究斑马鱼幼体明暗运动的实验方案。

Protocol for investigating light/dark locomotion in larval stage zebrafish using a standardized behavioral assay.

作者信息

Hillman Courtney, Kearn James, Parker Matthew O

机构信息

Surrey Sleep Research Centre, University of Surrey, GU2 7XH Guildford, UK.

Defence Science and Technology Laboratory (DSTL), Porton Down, SP4 0JQ Salisbury, UK.

出版信息

STAR Protoc. 2024 Dec 20;5(4):103346. doi: 10.1016/j.xpro.2024.103346. Epub 2024 Oct 10.

DOI:10.1016/j.xpro.2024.103346
PMID:39392745
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11735985/
Abstract

Zebrafish (Danio rerio) larvae offer a unique avenue for high-throughput in vivo investigation. The light/dark locomotor assay is widely used but lacks experimental consistency. Here, we present a protocol for a standardized light/dark assay by describing the steps for plating, acclimatizing larvae, performing the assay, and preparing drug exposure solutions. We also detail procedures for substance exposure and data analysis. For complete details on the use and execution of this protocol, please refer to Hillman et al..

摘要

斑马鱼（Danio rerio）幼体为高通量体内研究提供了一条独特途径。明暗运动分析被广泛使用，但缺乏实验一致性。在此，我们通过描述幼体接种、适应环境、进行分析以及制备药物暴露溶液的步骤，给出了标准化明暗分析的方案。我们还详细说明了物质暴露和数据分析的程序。有关本方案使用和执行的完整详细信息，请参考希尔曼等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/525f/11735985/72d690c870b0/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/525f/11735985/59a0b028b1e6/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/525f/11735985/bff9f47ad12d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/525f/11735985/f1c94ee8ea09/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/525f/11735985/7b75603f6619/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/525f/11735985/04b72fc725cb/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/525f/11735985/72d690c870b0/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/525f/11735985/59a0b028b1e6/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/525f/11735985/bff9f47ad12d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/525f/11735985/f1c94ee8ea09/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/525f/11735985/7b75603f6619/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/525f/11735985/04b72fc725cb/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/525f/11735985/72d690c870b0/gr5.jpg

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本文引用的文献

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A unified approach to investigating 4 dpf zebrafish larval behaviour through a standardised light/dark assay.通过标准化的光照/黑暗试验研究 4 dpf 斑马鱼幼虫行为的统一方法。
Prog Neuropsychopharmacol Biol Psychiatry. 2024 Aug 30;134:111084. doi: 10.1016/j.pnpbp.2024.111084. Epub 2024 Jul 11.
2
DMSO Concentrations up to 1% are Safe to be Used in the Zebrafish Embryo Developmental Toxicity Assay.高达1%的二甲基亚砜浓度用于斑马鱼胚胎发育毒性试验是安全的。
Front Toxicol. 2021 Dec 21;3:804033. doi: 10.3389/ftox.2021.804033. eCollection 2021.