Combined processing technologies: Promising approaches for reducing Allergenicity of food allergens.

Food allergy is a severe threat to human health. Although processing technologies are widely used to reduce allergenicity, hypoallergenic foods produced by a single processing technology cannot satisfy consumer demands. Combined processing technology (CPT) is a promising strategy for efficiently producing high-quality hypoallergenic foods. This paper reviews the effects of CPT on the allergenicity of food allergens from three aspects: physical-biochemical CPT, biochemical-biochemical CPT, and physical-physical CPT. The synergistic mechanisms, strengths, and limitations of these technologies were discussed. It was found that CPT is generally more effective than single-processing technologies. Physical-biochemical CPT is the most widely studied and well-established because physical and biochemical processing technologies complement each other and effectively disrupt conformational and linear epitopes. Biochemical-biochemical CPT primarily disrupts linear epitopes, but most methods are time-consuming. Physical-physical CPT is the least studied; they mainly disrupt conformational epitopes and only rarely affect linear epitopes.