Spivack J G, Maller J L
Mol Cell Biol. 1985 Dec;5(12):3629-33. doi: 10.1128/mcb.5.12.3629-3633.1985.
Microinjection of purified pp60v-src, the transforming protein of Rous sarcoma virus, into Xenopus laevis oocytes accelerated the rate of progesterone- or insulin-induced meiotic maturation. This acceleration was abolished by incubating the oocytes with cycloheximide or puromycin during a 2-h interval between pp60v-src microinjection and progesterone addition. In contrast, exposure to actinomycin D did not alter the acceleration of maturation by microinjected pp60v-src. Associated with progesterone treatment and pp60v-src microinjection were a number of qualitative changes in phosphoproteins; a few of these changes are common to both stimuli. These results indicate that the action of pp60v-src in oocytes involves both phosphorylation and protein synthetic events that affect oocyte maturation.
将劳氏肉瘤病毒的转化蛋白纯化的pp60v-src显微注射到非洲爪蟾卵母细胞中，可加速孕酮或胰岛素诱导的减数分裂成熟速率。在显微注射pp60v-src和添加孕酮之间的2小时间隔内，用环己酰亚胺或嘌呤霉素孵育卵母细胞可消除这种加速作用。相比之下，放线菌素D处理并不改变显微注射pp60v-src所导致的成熟加速。与孕酮处理和pp60v-src显微注射相关的是磷蛋白的一些定性变化；其中一些变化是两种刺激所共有的。这些结果表明，pp60v-src在卵母细胞中的作用涉及影响卵母细胞成熟的磷酸化和蛋白质合成事件。
