Key Laboratory of Applied Biology and Aquaculture of Northern Fishes in Liaoning Province, Dalian Ocean University, Dalian, China; College of Fisheries and Life Science, Dalian Ocean University, Dalian, China.
College of Environmental Sciences and Engineering, Dalian Maritime University, Dalian, China.
Fish Shellfish Immunol. 2024 Nov;154:109957. doi: 10.1016/j.fsi.2024.109957. Epub 2024 Oct 10.
The marbled flounder (Pseudopleuronectes yokohamae) is highly esteemed for its exceptional nutritional value and delicious taste. However, this species has extremely limited transcriptome data, which can offer priceless information for disease protection. In the study, the skin transcriptomic sequencing of P. yokohamae revealed 7.72 GB of clean data using the Nanopore sequencing platform. The results revealed 30,498 transcripts of functional annotations in the P. yokohamae transcriptome. All transcripts were searched in eight functional databases. A total of 10,337 ORFs were obtained, of which 6081 complete ORFs accounted for 58.83% of all predicted CDS. Moreover, 10,195 SSRs were detected. Meanwhile, the non-pecific immunity pathways were investigated for better understanding of the immunological reaction in P. yokohamae, and seven innate immune pathways were identified. The innate-immune related genes were highly expressed in the NOD-like receptor signaling pathway, followed by the C-type lectin receptor signaling pathway, Toll-like receptor signaling pathway, RIG-I-like receptor signaling pathway and Cytosolic DNA-sensing pathway. In this study, four families of antimicrobial peptides (AMPs) in P. yokohamae were analysed for the first time, including piscidins, hepcidins, liver-expressed antimicrobial peptide and defensins. Seven AMPs, including Pypleurocidin-like WF3, Pypleurocidin-like WFX, Pyhepcidin 1, Pyhepcidin-like 1, PyLEAP-2, Pybeta-defensin and Pybeta-defensin-like 1, were further identified. The seven AMPs showed a highly identity in their cDNA and genomic structures and an inducible expression pattern preferable to skin in response to pathogens. The transcriptomic data and investigation of AMPs from P. yokohamae promote a deeper awareness of fish mucosal immunity and provide information in the prevention of fish diseases.
黄斑比目鱼（Pseudopleuronectes yokohamae）因其极高的营养价值和美味口感而备受推崇。然而，该物种的转录组数据极为有限，这些数据可以为疾病保护提供无价的信息。在本研究中，使用 Nanopore 测序平台对 P. yokohamae 的皮肤转录组进行测序，获得了 7.72GB 的清洁数据。结果显示，P. yokohamae 转录组中具有 30498 个功能注释的转录本。所有的转录本都在八个功能数据库中进行了搜索。总共获得了 10337 个 ORF，其中 6081 个完整的 ORF 占所有预测 CDS 的 58.83%。此外，还检测到了 10195 个 SSRs。同时，为了更好地了解 P. yokohamae 的免疫反应，研究了非特异性免疫途径，共鉴定出 7 条先天免疫途径。在 NOD 样受体信号通路中，先天免疫相关基因高度表达，其次是 C 型凝集素受体信号通路、Toll 样受体信号通路、RIG-I 样受体信号通路和细胞质 DNA 感应通路。在本研究中，首次分析了 P. yokohamae 中的四种抗菌肽（AMPs）家族，包括鱼精蛋白、hepcidins、肝表达抗菌肽和防御素。进一步鉴定了 7 种 AMPs，包括 Pypleurocidin-like WF3、Pypleurocidin-like WFX、Pyhepcidin 1、Pyhepcidin-like 1、PyLEAP-2、Pybeta-defensin 和 Pybeta-defensin-like 1。这 7 种 AMPs 在 cDNA 和基因组结构上具有高度的同一性，并表现出对病原体的诱导表达模式，首选皮肤。来自 P. yokohamae 的转录组数据和 AMP 研究促进了对鱼类黏膜免疫的更深层次认识，并为鱼类疾病的预防提供了信息。
