College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; National Engineering Research Center for Fruit and Vegetable Processing, Key Lab of Fruit and Vegetable Processing, Ministry of Agriculture, Beijing Key Laboratory of Food Non-Thermal Processing, Beijing, 100083, China.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; National Engineering Research Center for Fruit and Vegetable Processing, Key Lab of Fruit and Vegetable Processing, Ministry of Agriculture, Beijing Key Laboratory of Food Non-Thermal Processing, Beijing, 100083, China.
Int J Biol Macromol. 2024 Nov;281(Pt 4):136462. doi: 10.1016/j.ijbiomac.2024.136462. Epub 2024 Oct 10.
This study investigated the impact of high-pressure processing (HPP) treatment on the structure and physicochemical properties of pectin (PEC)/carboxymethyl cellulose (CMC) composite films, along with the development of new active films incorporating emodin as an antibacterial agent. The results showed that 500 MPa/20 min HPP treatment significantly improved the tensile strength (from 45.91 ± 4.63 MPa to 52.24 ± 4.87 MPa) and elongation at the break (from 5.00 ± 1.44 % to 11.72 ± 2.97 %) of the films. It also improved the film's thermal stability and had no significant effect on its thermal degradability. Moreover, emodin was incorporated into the PEC/CMC film-forming solution and subjected to 500 MPa/20 min HPP treatment to investigate the structure, functional properties, optical properties, and antibacterial activity of the film. The emodin caused the film structural alteration, but significantly improved the water vapor barrier properties. It also reduced the film brightness and light transmission. The antibacterial assessment demonstrated that the film's antibacterial activity was correlated positively with increasing emodin content, and the number of viable cells of Staphylococcus aureus decreased by 1.29 log CFU/mL, 1.70 log CFU/mL, and 1.80 log CFU/mL with different levels of EM antimicrobial films after 12 h.
本研究考察了高压处理（HPP）对果胶（PEC）/羧甲基纤维素（CMC）复合膜结构和物理化学性质的影响，以及开发新的含有大黄素作为抗菌剂的活性膜。结果表明，500 MPa/20 min 的 HPP 处理显著提高了薄膜的拉伸强度（从 45.91 ± 4.63 MPa 提高到 52.24 ± 4.87 MPa）和断裂伸长率（从 5.00 ± 1.44%提高到 11.72 ± 2.97%）。它还提高了薄膜的热稳定性，对其热降解性没有显著影响。此外，将大黄素掺入 PEC/CMC 成膜溶液中，并进行 500 MPa/20 min 的 HPP 处理，以研究薄膜的结构、功能特性、光学性质和抗菌活性。大黄素引起了薄膜结构的改变，但显著提高了水蒸气阻隔性能。它还降低了薄膜的亮度和透光率。抗菌评估表明，薄膜的抗菌活性与大黄素含量的增加呈正相关，金黄色葡萄球菌的活菌数在 12 小时后分别减少了 1.29 log CFU/mL、1.70 log CFU/mL 和 1.80 log CFU/mL。
