Université de Caen Normandie, CNRS, Normandie Université, ISTCT UMR6030, GIP Cyceron, Caen, France.
Department of Oncology UNIL CHUV, University of Lausanne, Lausanne, Switzerland; Ludwig Institute for Cancer Research, University of Lausanne, Lausanne, Switzerland.
Methods Cell Biol. 2024;189:135-152. doi: 10.1016/bs.mcb.2024.06.004. Epub 2024 Jul 9.
In the context of high-grade gliomas such as glioblastoma (GBM), the immune part of the tumor microenvironment (TME) is involved in tumor growth and tumor recurrence. It is mostly represented by high amount of macrophages and low amount of lymphocytes. GBM in itself as well as x-ray-based radiotherapy, a standard treatment for brain tumors, are also associated with systemic effects like lymphopenia that correlates with a poor prognosis. This contributes to the immune-suppressive nature of the TME and may explain the lack of the anti-tumor immune response. Radiation-induced lymphopenia (RIL) is generally evaluated on CD4 and CD8 count or on a CBC (complete blood count), but the heterogeneity of the subtypes prompts us to explore them in detail to better understand the cellular response to brain irradiation. To facilitate and develop the evaluation of x-ray brain exposure on circulating immune cells, we developed a reproducible and reliable method to quantify the variation of lymphoid and myeloid subtypes using flow cytometry after brain irradiation in the rodent.
在高级别神经胶质瘤（如胶质母细胞瘤 [GBM]）的情况下，肿瘤微环境（TME）中的免疫部分参与肿瘤生长和肿瘤复发。它主要由大量的巨噬细胞和少量的淋巴细胞组成。GBM 本身以及基于 X 射线的放射疗法，是脑肿瘤的标准治疗方法，也与全身性效应相关，如与预后不良相关的淋巴细胞减少症。这导致了 TME 的免疫抑制性质，并可能解释了缺乏抗肿瘤免疫反应的原因。放射诱导的淋巴细胞减少症（RIL）通常通过 CD4 和 CD8 计数或全血细胞计数（CBC）来评估，但亚型的异质性促使我们详细探索它们，以更好地了解对脑照射的细胞反应。为了促进和发展对循环免疫细胞的 X 射线脑暴露的评估，我们开发了一种可重复且可靠的方法，使用流式细胞术在啮齿动物中进行脑照射后定量分析淋巴样和髓样亚型的变化。
