Department of Orthopedics, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
Zhejiang Key Laboratory of Precision Psychiatry, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
Adv Mater. 2024 Nov;36(48):e2406891. doi: 10.1002/adma.202406891. Epub 2024 Oct 12.
Suspension bath-based 3D bioprinting (SUB3BP) is effective in creating engineered vascular structures. The transfer of oxygen and nutrients via engineered vascular networks is necessary for tissue or organ survival and integration following transplantation. Existing SUB3BP techniques face challenges in fabricating hierarchical structures with multicellular organization, including issues related to suspension bath removal, restricted material choices, and low accuracy. A next-generation SUB3BP technique that is removal-free and multicellular is presented. A simple, storable, stable, and scalable starch hydrogel design leverages the diverse spectrum of hydrogels available for use in SUB3BP. Starch granules (8.1 µm) create vascular structures with minimal surface roughness (2.5 µm) that simulate more natural vessel walls compared to prior research. The development of cells and organoids, as well as the bioprinting of multicellular skin models with vasculature, demonstrates that starch suspension baths eliminate the removal process and have the potential for fabricating artificial tissue with a hierarchical structure and multicellular distribution.
基于悬浮浴的 3D 生物打印（SUB3BP）在构建工程血管结构方面非常有效。在移植后，工程化的血管网络用于组织或器官的存活和整合，以实现氧气和营养物质的传输。现有的 SUB3BP 技术在制造具有细胞多组织的层次结构方面面临挑战，包括与悬浮浴去除、材料选择受限和精度低相关的问题。提出了一种无悬浮浴去除和多细胞的下一代 SUB3BP 技术。一种简单、可储存、稳定且可扩展的淀粉水凝胶设计利用了 SUB3BP 中可用的各种水凝胶。淀粉颗粒（8.1μm）制造出的血管结构具有最小的表面粗糙度（2.5μm），与之前的研究相比，更能模拟天然血管壁。细胞和类器官的发育以及具有血管的多细胞皮肤模型的生物打印表明，淀粉悬浮浴消除了去除过程，并有可能制造出具有层次结构和多细胞分布的人工组织。
