Sukumar Kalpana, Bharathi Muruganantham, Hirad Abdurahman Hajinur, Alarfaj Abdullah A, Hussein-Al-Ali Samer Hasan, Surya Parthasarathy
Department of Physics, Saveetha Engineering College, Saveetha Nagar, Thandalam, Chennai, 602105, India.
Centre for Drug Discovery, Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, 641021, India.
Chem Biodivers. 2025 Feb;22(2):e202401817. doi: 10.1002/cbdv.202401817. Epub 2024 Dec 3.
Innovative drug delivery platforms for selective, regulated, and sustained release of anticancer drugs are crucial in cancer treatment. This study presents nanoparticles developed from chitosan (CS), graphene oxide (GO), and magnetite (FeO), and their nanocomposites to enhance the loading and release efficiency of camptothecin (CPT). Nanostructures were characterized using imaging microscopy, FT-IR, and X-ray diffraction, with an average crystallite size of 5.5 nm. Camptothecin binding proportions were 70 % for CS, 81 % for CS@FeO, 58 % for CS@GO, and 74 % for CS@GO/FeO. At pH 5.0, CPT release ratios were 87 %, 80 %, 88 %, and 90 %, respectively, and at pH 7.4, 84 %, 72 %, 89 %, and 87 %. Cytotoxicity was assessed using the MTT assay against HepG2 and SMMC-7721 cancer cells. CPT-CS@GO/FeO exhibited the highest survival at 5 μM and 12.5 μM concentrations, indicating it as the most effective nanocarrier for camptothecin delivery. The study demonstrates CS@GO/FeO's potential as a superior drug delivery system.
用于抗癌药物选择性、可控性和持续释放的创新药物递送平台在癌症治疗中至关重要。本研究展示了由壳聚糖（CS）、氧化石墨烯（GO）和磁铁矿（FeO）及其纳米复合材料制备的纳米颗粒，以提高喜树碱（CPT）的负载和释放效率。使用成像显微镜、傅里叶变换红外光谱（FT-IR）和X射线衍射对纳米结构进行了表征，平均微晶尺寸为5.5纳米。喜树碱与CS的结合比例为70%，与CS@FeO的结合比例为81%，与CS@GO的结合比例为58%，与CS@GO/FeO的结合比例为74%。在pH 5.0时，CPT的释放率分别为87%、80%、88%和90%，在pH 7.4时，释放率分别为84%、72%、89%和87%。使用MTT法对HepG2和SMMC-7721癌细胞进行细胞毒性评估。CPT-CS@GO/FeO在5 μM和12.5 μM浓度下表现出最高的存活率，表明它是喜树碱递送最有效的纳米载体。该研究证明了CS@GO/FeO作为一种卓越药物递送系统的潜力。
