使用 NanoBiT 发光生物传感器实时测量 IP3 的产生。
Measuring IP3 Generation in Real-Time Using a NanoBiT Luminescence Biosensor.
机构信息
Metabolism and Systems Science and Centre for Diabetes, Endocrinology and Metabolism (CEDAM), University of Birmingham, Birmingham, UK.
Centre for Membrane Proteins and Receptors (COMPARE), Universities of Birmingham and Nottingham, Birmingham, UK.
出版信息
Methods Mol Biol. 2025;2861:33-42. doi: 10.1007/978-1-0716-4164-4_3.
G protein-coupled receptors that activate Gq/11 regulate a range of physiological processes including neurotransmission, energy homeostasis, blood pressure regulation, and calcium homeostasis. Activation of Gq/11-coupled receptors stimulates the generation of inositol 1,4,5-trisphosphate (IP3), which mobilizes intracellular calcium release from the endoplasmic reticulum. This chapter describes an assay that uses a NanoBiT-IP3 luminescent biosensor to detect increases in IP3 in live cells. It describes how to perform these assays to assess signaling by the ghrelin receptor and the calcium-sensing receptor in HEK293 cells.
G 蛋白偶联受体激活 Gq/11 调节多种生理过程，包括神经递质传递、能量平衡、血压调节和钙平衡。Gq/11 偶联受体的激活刺激肌醇 1,4,5-三磷酸 (IP3) 的产生，从而从内质网中动员细胞内钙释放。本章描述了一种使用 NanoBiT-IP3 发光生物传感器检测活细胞中 IP3 增加的测定法。它描述了如何进行这些测定以评估 ghrelin 受体和钙敏感受体在 HEK293 细胞中的信号传递。
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