State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Food Chem. 2025 Feb 1;464(Pt 1):141541. doi: 10.1016/j.foodchem.2024.141541. Epub 2024 Oct 5.
Breast milk (BM) fulfills the nutritional needs of infants and sets the standard for infant formula (IF). However, profiling the differential phosphoproteome between BM and IF remains unclear. Herein, a titanium (IV) (Ti)-immobilized magnetic nanoplatform (FeO@GO@PDA-Ti) was constructed by self-assembly polymerization of dopamine on magnetic graphene oxide, followed by immobilizing Ti through chelation for phosphopeptide enrichment. FeO@GO@PDA-Ti possessed outstanding selectivity (1/1000, a molar ratio of β-casein digests to bovine serum albumin digests) and favorable sensitivity (2.5 fmol/μL), along with rapid magnetic separation. Excellent phosphopeptide capture efficiencies were obtained for BM and IF using FeO@GO@PDA-Ti as an adsorbent coupled with liquid chromatography-mass spectrometry/mass spectrometry. There were 191 and 239 phosphopeptides found in BM and IF, respectively, with 36 phosphoproteins identified in both. However, BM and IF shared only 17 phosphopeptides and 4 phosphoproteins. The variation in the phosphoproteome between BM and IF provides valuable insights into the optimization of IF humanization.
母乳（BM）满足了婴儿的营养需求，并为婴儿配方奶粉（IF）设定了标准。然而，母乳和 IF 之间差异磷酸化蛋白质组的分析仍不清楚。本研究通过多巴胺在磁性氧化石墨烯上的自组装聚合，构建了一种钛（IV）（Ti）固定化磁性纳米平台（FeO@GO@PDA-Ti），随后通过螯合作用将 Ti 固定化用于磷酸肽富集。FeO@GO@PDA-Ti 具有出色的选择性（1/1000，β-酪蛋白消化物与牛血清白蛋白消化物的摩尔比）和良好的灵敏度（2.5 fmol/μL），同时具有快速的磁分离性能。使用 FeO@GO@PDA-Ti 作为吸附剂，结合液相色谱-质谱/质谱法，从 BM 和 IF 中获得了优异的磷酸肽捕获效率。在 BM 和 IF 中分别发现了 191 种和 239 种磷酸肽，其中 36 种磷酸蛋白在两者中均有鉴定。然而，BM 和 IF 仅共享 17 种磷酸肽和 4 种磷酸蛋白。BM 和 IF 之间磷酸化蛋白质组的差异为 IF 人源化的优化提供了有价值的见解。
