Division of Microbiology and Biotechnology, Yenepoya Research Centre, Yenepoya (Deemed to be University), University Road, Deralakatte, Mangalore, 575018, India.
Department of Pediatrics, Yenepoya Medical College, Yenepoya (Deemed to be University), Deralakatte, Mangalore, 575018, India.
Diagn Microbiol Infect Dis. 2024 Dec;110(4):116552. doi: 10.1016/j.diagmicrobio.2024.116552. Epub 2024 Oct 5.
Neonatal sepsis is a significant problem in developing nations, but current gold-standard diagnostic methods, such as blood culture, is slow and time-consuming. Here, we describe the development of a colorimetric loop-mediated isothermal amplification (LAMP) assay that targets the wabG gene in the lipopolysaccharide region of K. pneumoniae, offering a limit of detection (LOD) of 40 CFU/ml with specificity for K. pneumoniae compared to other non-Klebsiella strains. The sensitivity and specificity of the LAMP assay were found to be 90 % and 100 %, respectively, with a positive predictive value of 100 % and a negative predictive value of 96.47 %. The LAMP assay demonstrated a significantly shorter turnaround time of 1 h. The LAMP assay was found to be simpler, quicker, and more sensitive than traditional detection techniques such as PCR and blood culture.
新生儿败血症在发展中国家是一个重大问题，但目前的金标准诊断方法，如血液培养，既缓慢又耗时。在这里，我们描述了一种比色环介导的等温扩增 (LAMP) 检测方法的开发，该方法针对肺炎克雷伯菌脂多糖区域的 wabG 基因，其检测限 (LOD) 为 40 CFU/ml，与其他非肺炎克雷伯菌菌株相比具有特异性。LAMP 检测方法的灵敏度和特异性分别为 90%和 100%，阳性预测值为 100%，阴性预测值为 96.47%。LAMP 检测方法的检测周转时间明显更短，为 1 小时。与传统的检测技术，如 PCR 和血液培养相比，LAMP 检测方法更简单、更快、更灵敏。
