Leibniz Institute for Zoo and Wildlife Research (IZW), Berlin, Germany.
Faculty Veterinary Medicine, Freie Universitaet Berlin, Berlin, Germany.
Reprod Domest Anim. 2024 Oct;59 Suppl 3:e14700. doi: 10.1111/rda.14700.
A new synergistic approach of classical conservation strategies combined with advanced assisted reproduction technologies (aART) allows for protection and rescue of endangered keystone species at the brink of extinction, which can help to safeguard complex ecosystems. Reproduction biology and management in mammal species is not only challenging in regards to their diverging sizes, anatomy, and often unknown physiology; it also requires customized training or chemical restraint protocols for safe handling. Besides these general challenges, there are several new assisted reproduction techniques (ART) specifically tailored to critically endangered mammals. The current portfolio of ART in these mammalian taxa is ranging from sexual cycle characterization and manipulation, semen collection and cryopreservation, artificial insemination, biobanking of living cells, oocyte collection, in vitro fertilization (IVF), and embryo production, embryo transfer as well as stem cell-derived in vitro gametogenesis for generating gametes in culture. The article covers advanced assisted reproduction technologies (aART), success and challenges, as well as ethical implications.
一种新的协同方法，将经典的保护策略与先进的辅助生殖技术（aART）相结合，可用于保护和拯救处于灭绝边缘的关键濒危物种，有助于保护复杂的生态系统。哺乳动物物种的繁殖生物学和管理不仅在体型、解剖结构上存在差异，而且通常未知的生理学方面具有挑战性；还需要针对安全处理制定定制的培训或化学约束协议。除了这些一般挑战外，还有一些专门针对极度濒危哺乳动物的新辅助生殖技术（ART）。目前，这些哺乳动物分类群的 ART 组合包括对性周期的特征和操作、精液收集和冷冻保存、人工授精、活细胞的生物库、卵母细胞收集、体外受精（IVF）和胚胎生产、胚胎移植以及干细胞衍生的体外配子发生，以在培养中生成配子。本文涵盖了先进的辅助生殖技术（aART）、成功与挑战以及伦理问题。
