Sanya Nanfan Research Institute of Hainan University, Hainan University, Sanya, China.
Hainan Research Academy of Environmental Sciences, Haikou, China.
Sci Rep. 2024 Oct 13;14(1):23909. doi: 10.1038/s41598-024-72796-z.
Land use changes soil microbial and chemical properties, but the mechanism of biological nitrogen fixation under different land use patterns is rarely reported, so we used four types of soil: Natural forest soil (NS), healthy banana soil (HS), diseased banana soil (DS) and paddy soil (PS). Treatments included the control (CK), addition of glucose (G), addition of glucose and ammonium nitrate (GN), addition of banana straw (BS), addition of banana straw and ammonium nitrate (BSN), addition of banana root (BR), and addition of banana root and ammonium nitrate (BRN). The study found that the change of soil utilization types, glucose addition increased carbon dioxide emissions (Compared with the control, increased by 963.11%, 508.39%, 794.77% and 511.34%, respectively) and enhanced the ability of soil microbial nitrogen fixation. Importantly, natural forest soil microorganisms have a higher biological nitrogen fixation capacity compared to other types of soils. Glucose addition caused the accumulation of ammonium nitrogen (Compared with the control, increased by 426.08%, 934.21%, 420% and 1065.95%, respectively), indicating that microorganisms had higher utilization efficiency of soluble carbon and enhanced the biological nitrogen fixation capacity, and nitrogen addition caused the accumulation of ammonium nitrogen, thereby weakening the biological nitrogen fixation capacity. At the same time, glucose significantly increased the Fimicutes phylum (83.73%, 66.38%, 67.18% and 70.36%) and lowered the level of other bacterial phylums, thereby reducing the bacterial network structure, and the stability of the soil environment has decreased. Forest analysis showed that CO was an important factor in predicting the bacterial community structure of different soil types, an increase in CO content can predict drastic changes in the bacterial community. Bacteria at the Fimicutes phylum level preferred glucose, which may also have a negative effect on bacteria at the level of other phylums.
土地利用方式的改变会影响土壤微生物和化学性质，但不同土地利用方式下生物固氮的机制却鲜有报道，因此我们选用了四种土壤：天然林土壤（NS）、健康香蕉土壤（HS）、患病香蕉土壤（DS）和稻田土壤（PS）。处理方式包括对照（CK）、添加葡萄糖（G）、添加葡萄糖和硝酸铵（GN）、添加香蕉秸秆（BS）、添加香蕉秸秆和硝酸铵（BSN）、添加香蕉根（BR）和添加香蕉根和硝酸铵（BRN）。研究发现，土壤利用类型的变化、葡萄糖的添加增加了二氧化碳的排放（与对照相比，分别增加了 963.11%、508.39%、794.77%和 511.34%），增强了土壤微生物固氮能力。重要的是，与其他类型的土壤相比，天然林土壤中的微生物具有更高的生物固氮能力。葡萄糖的添加导致铵态氮的积累（与对照相比，分别增加了 426.08%、934.21%、420%和 1065.95%），这表明微生物对可溶性碳具有更高的利用效率，增强了生物固氮能力，而氮的添加导致铵态氮的积累，从而削弱了生物固氮能力。同时，葡萄糖显著增加了厚壁菌门（83.73%、66.38%、67.18%和 70.36%），降低了其他细菌门的水平，从而减少了细菌网络结构，土壤环境的稳定性降低。森林分析表明，CO 是预测不同土壤类型细菌群落结构的重要因素，CO 含量的增加可以预测细菌群落的剧烈变化。厚壁菌门水平的细菌更喜欢葡萄糖，这也可能对其他门水平的细菌产生负面影响。
