Li Shanshan, Lu Mingzhu, Dai Chenxi, Xu Bolong, Wu Nier, Wang Lin, Liu Chao, Chen Fangzhou, Yang Haokun, Huang Zhijun, Liu Huiyu, Zhou Dongsheng
Beijing Advanced Innovation Center for Soft Matter Science and Engineering, State Key Laboratory of Organic-Inorganic Composites, Bionanomaterials & Translational Engineering Laboratory, Beijing Key Laboratory of Bioprocess, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, China.
State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, 100071, China.
Small. 2025 Feb;21(6):e2407180. doi: 10.1002/smll.202407180. Epub 2024 Oct 13.
With the increasing spread of multidrug-resistant (MDR) bacteria worldwide, it is needed to develop antibiotics-alternative strategies for the treatment of bacterial infections. This work develops a multifunctional single-component palladium nanosheet (PdNS) with broad-spectrum and highly effective bactericidal activity against MDR bacteria. PdNS exerts its endogenous nanoknife (mechanical cutting) effect and peroxidase-like activity independent of light. Under near-infrared region (NIR) light irradiation, PdNS exhibits photothermal effect to produce local heat and meanwhile possesses photodynamic effect to generate O; notably, PdNS has catalase-like activity-dependent extra photodynamic effect upon HO addition. PdNS+HO+NIR employs a collectively synergistic mechanism of nanoknife effect, peroxidase/catalase-like catalytic activity, photothermal effect, and photodynamic effect for bacterial killing. PdNS+HO+NIR causes compensatory elevated phospholipid biosynthesis, disordered energy metabolism, increased cellular ROS levels and excessive oxidative stress, and inhibited nucleic acid synthesis in bacteria. In mice, PdNS+HO+NIR gives >92.7% bactericidal rates at infected wounds and almost the full recovery of infected wounds, and it leads to extensive down-regulation of proinflammatory pathways and comprehensive up-regulation of wound healing pathways, conferring elevated inflammation resolution and meanwhile accelerated wound repair. PdNS+HO+NIR represents a highly efficient nanoplatform for photoenhanced treatment of superficial infections.
随着耐多药（MDR）细菌在全球范围内的日益传播，需要开发抗生素替代策略来治疗细菌感染。这项工作开发了一种多功能单组分钯纳米片（PdNS），它对MDR细菌具有广谱且高效的杀菌活性。PdNS发挥其独立于光的内源性纳米刀（机械切割）效应和过氧化物酶样活性。在近红外（NIR）光照射下，PdNS表现出光热效应以产生局部热量，同时具有光动力效应以产生单线态氧；值得注意的是，在添加过氧化氢后，PdNS具有依赖于过氧化氢酶样活性的额外光动力效应。PdNS+过氧化氢+NIR采用纳米刀效应、过氧化物酶/过氧化氢酶样催化活性、光热效应和光动力效应的协同机制来杀灭细菌。PdNS+过氧化氢+NIR导致细菌中磷脂生物合成代偿性升高、能量代谢紊乱、细胞活性氧水平增加和过度氧化应激，并抑制核酸合成。在小鼠中，PdNS+过氧化氢+NIR在感染伤口处的杀菌率>92.7%，感染伤口几乎完全愈合，并且它导致促炎途径广泛下调和伤口愈合途径全面上调，从而提高炎症消退并同时加速伤口修复。PdNS+过氧化氢+NIR代表了一种用于光增强治疗浅表感染的高效纳米平台。
