Severina L O, Bashkatova N A
Mikrobiologiia. 1979 Nov-Dec;48(6):994-8.
Various methods have been tested in order to isolate endolipase from the cells of Serratia marcescens 345: cell disintegration by ultrasound and French press as well as incubation of the cellular suspension with sodium salts of bile acids. The combined effect of ultrasound and these salts on the cellular suspension of S. marcescens 345 was also studied. The highest lipolytic activity was found in the supernatant obtained upon incubation of the cells with sodium cholate at a concentration of 0.8% for 30 min.
为了从粘质沙雷氏菌345细胞中分离出内脂酶，已经测试了各种方法：通过超声和法国压榨机进行细胞破碎，以及将细胞悬浮液与胆汁酸钠盐一起孵育。还研究了超声和这些盐对粘质沙雷氏菌345细胞悬浮液的联合作用。在细胞与浓度为0.8%的胆酸钠孵育30分钟后获得的上清液中发现了最高的脂解活性。
