Milićević Vesna, Glišić Dimitrije, Veljović Ljubiša, Zurovac Sapundžić Zorana, Šolaja Sofija, Jezdimirović Nemanja
Virology Department, Institute of Veterinary Medicine of Serbia, Belgrade, Serbia.
J Vet Diagn Invest. 2025 Jan;37(1):145-147. doi: 10.1177/10406387241280629. Epub 2024 Oct 14.
To prevent significant economic losses, some countries have successfully eradicated enzootic bovine leukosis (EBL), which is caused by bovine leukemia virus (BLV) infection. In Serbia, efforts to eliminate EBL commenced in the late 1990s. Recognizing the disparities in test selection among laboratories and variations in quality, we evaluated the diagnostic sensitivity and specificity of commercial ELISAs using field samples in Serbia. Using 5 commercial ELISA kits, we tested 138 cattle serum samples, submitted for confirmatory testing between 2020 and 2023, along with 100 serum samples from BLV-negative herds. We found 100% agreement of the ID Screen BLV Competition (IDvet), Svanovir BLV gp51-Ab (Svanova), and INgezim BLV Compac 2.0 (Ingenasa) ELISAs. We observed 93% agreement comparing these 3 kits to the Bovine Leukemia Virus Antibody test kit (VMRD). Agreements of 92% and 88.4% were determined between Idexx and IDvet, Svanova, and Ingenasa kits, and between Idexx and VMRD kits, respectively.
为防止重大经济损失，一些国家已成功根除由牛白血病病毒（BLV）感染引起的地方流行性牛白血病（EBL）。在塞尔维亚，消除EBL的工作始于20世纪90年代末。认识到各实验室在检测方法选择上的差异以及质量的变化，我们使用塞尔维亚的现场样本评估了商用ELISA的诊断敏感性和特异性。我们使用5种商用ELISA试剂盒，检测了2020年至2023年间提交进行确证检测的138份牛血清样本，以及来自BLV阴性牛群的100份血清样本。我们发现ID Screen BLV Competition（IDvet）、Svanovir BLV gp51-Ab（Svanova）和INgezim BLV Compac 2.0（Ingenasa）ELISA试剂盒的结果完全一致。将这3种试剂盒与牛白血病病毒抗体检测试剂盒（VMRD）进行比较时，我们观察到一致性为93%。Idexx试剂盒与IDvet、Svanova和Ingenasa试剂盒之间的一致性分别为92%和88.4%，Idexx试剂盒与VMRD试剂盒之间的一致性为88.4%。
